Vmax™ X2 Chemically Competent Cells
  • Vmax™ X2 Chemically Competent Cells

Vmax™ X2 Chemically Competent Cells

Cat No. Size Price Quantity
CL1300-05 5 rxn $72.00
CL1300-10 10 rxn $137.00
CL1300-20 20 rxn $256.00
Lead time: 2-4 business days

Description

Vmax™ X2 is a novel, fast-growing bacterial strain designed and optimized for high-level recombinant protein expression. Vmax™ cells are derived from the gram-negative, low endotoxin, non-pathogenic marine bacterium, Vibrio natriegens. With a doubling time twice that of E. coli and the ability to generate greater amounts of biomass per volume of cells, Vmax X2 results in larger amounts of soluble protein faster. Vmax™ X2 cells are compatible with plasmids and antibiotics commonly used with bacterial expression systems such as E. coli BL21(DE3) and has a transformation efficiency rivaling the best alternative bacterial expression strains. Built with a tightly controlled, IPTG-inducible T7 promoter system, Vmax™ X2 cells can be cultured using routine growth medium, as well as commercial auto-induction media, or our Vmax™ enriched growth media (recommended for rapid growth and greatest accumulation of biomass). In addition, Vmax™ cells can be induced over a wide range of time points, from an OD600 of 0.1 to over 1.0, without a loss of final protein yield, dramatically increasing workflow flexibility.

Quick Reference Manual for Vmax™ Express Chemically Competent CellsDownload the Quick Reference Manual for Vmax™ X2 Chemically Competent Cells.

Instructions Manual for Vmax™ Express Chemically Competent CellsDownload the User Guide for Vmax™ X2 Chemically Competent Cells.

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Vmax™ X2 is a novel, fast-growing bacterial strain designed and optimized for high-level recombinant protein expression. Vmax™ cells are derived from the gram-negative, low endotoxin, non-pathogenic marine bacterium, Vibrio natriegens. With a doubling time twice that of E. coli and the ability to generate greater amounts of biomass per volume of cells, Vmax X2 results in larger amounts of soluble protein faster. Vmax™ X2 cells are compatible with plasmids and antibiotics commonly used with bacterial expression systems such as E. coli BL21(DE3) and has a transformation efficiency rivaling the best alternative bacterial expression strains. Built with a tightly controlled, IPTG-inducible T7 promoter system, Vmax™ X2 cells can be cultured using routine growth medium, as well as commercial auto-induction media, or our Vmax™ enriched growth media (recommended for rapid growth and greatest accumulation of biomass). In addition, Vmax™ cells can be induced over a wide range of time points, from an OD600 of 0.1 to over 1.0, without a loss of final protein yield, dramatically increasing workflow flexibility.

Quick Reference Manual for Vmax™ Express Chemically Competent CellsDownload the Quick Reference Manual for Vmax™ X2 Chemically Competent Cells.

Instructions Manual for Vmax™ Express Chemically Competent CellsDownload the User Guide for Vmax™ X2 Chemically Competent Cells.

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Product Details

Name Vmax™ Express - Chemically Competent Cells
Cat. No. CL1200
Alternative Names
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.