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C57Bl/6 bone marrow cells were stained with 0.25 ug violetFluor™ 450 Anti-Mouse Ly-6G (75-1276) (right panel) or 0.25 ug violetFluor™ 450 Rat IgG2a isotype control (left panel).
  • C57Bl/6 bone marrow cells were stained with 0.25 ug violetFluor™ 450 Anti-Mouse Ly-6G (75-1276) (right panel) or 0.25 ug violetFluor™ 450 Rat IgG2a isotype control (left panel).

violetFluor™ 450 Anti-Mouse Ly-6G (1A8)

Cat No. Size Price Quantity
75-1276-U025 25 µg $60.00
75-1276-U100 100 µg $141.00

Description

The 1A8 antibody binds to mouse Ly-6G, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Ly-6G is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils.

In the mouse, the 1A8 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes. Note: for identification of Ly-C, an alternative antibody, clone RB6-8C5, has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C.

 

Recent Publications:

Lei Y, Guerra Martinez C, Torres-Odio S, et al. Elevated type I interferon responses potentiate metabolic dysfunction, inflammation, and accelerated aging in mtDNA mutator mice. Sci Adv. 2021 May 26;7(22):eabe7548. doi: 10.1126/sciadv.abe7548. PMID: 34039599.

» View on PubMed

Li Y, Han Y, Su R, Liu Y, Chong G, Xu D, He R, Liu Y, Dong H, Shi D, Li Y. Photosensitizer-Laden Neutrophils Are Controlled Remotely for Cancer Immunotherapy. Cell Rep. 2020 Dec 15;33(11):108499. doi: 10.1016/j.celrep.2020.108499. PMID: 33326787. 

» View on ScienceDirect

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The 1A8 antibody binds to mouse Ly-6G, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Ly-6G is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils.

In the mouse, the 1A8 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes. Note: for identification of Ly-C, an alternative antibody, clone RB6-8C5, has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C.

 

Recent Publications:

Lei Y, Guerra Martinez C, Torres-Odio S, et al. Elevated type I interferon responses potentiate metabolic dysfunction, inflammation, and accelerated aging in mtDNA mutator mice. Sci Adv. 2021 May 26;7(22):eabe7548. doi: 10.1126/sciadv.abe7548. PMID: 34039599.

» View on PubMed

Li Y, Han Y, Su R, Liu Y, Chong G, Xu D, He R, Liu Y, Dong H, Shi D, Li Y. Photosensitizer-Laden Neutrophils Are Controlled Remotely for Cancer Immunotherapy. Cell Rep. 2020 Dec 15;33(11):108499. doi: 10.1016/j.celrep.2020.108499. PMID: 33326787. 

» View on ScienceDirect

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Product Details

Name violetFluor™ 450 Anti-Mouse Ly-6G (1A8)
Cat. No. 75-1276
Alternative Names
Gene ID 546644
Clone 1A8
Isotype Rat IgG2a, κ
Reactivity Mouse
Cross Reactivity
Format violetFluor™ 450
Application Flow Cytometry
Citations*

Shindo R, Katagiri T, Komazawa-Sakon S, et al. Regenerating islet-derived protein (Reg)3β plays a crucial role in attenuation of ileitis and colitis in mice. Biochem Biophys Rep. 2020;21:100738. Published 2020 Feb 7. doi:10.1016/j.bbrep.2020.100738.

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Kamaly N, Fredman G, Subramanian M, Gadde S, Pesic A, Cheung L, Fayad ZA, Langer R, Tabas I, and Cameron O. 2013. Proc. Natl. Acad. Sci. 110. 6506-6511. (flow cytometry).

Stinn W, Buettner A, Weiler H, Friedrichs B, Luetjen S, van Overveld F, Meurrens K, Janssens K, Gebel S, Stabbert R, and Haussman H-J. 2013. Toxicol. Sci. 131: 596-611 (flow cytometry).

Enoksson M, Moller-Westerberg C, Wicher G, Fallon PG, Forsberg-Nilsson K, Lunderius-Andersson C, and Nilsson G. 2013. Blood. 121. 530-536 (flow cytometry).

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.