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C57Bl/6 bone marrow cells were stained with PerCP-Cy5.5 Anti-Mouse CD11b (65-0112) and 0.5 ug violetFluor™ 450 Anti-Mouse F4/80 Antigen (75-4801) (right panel) or 0.5 ug violetFluor™ 450 Rat IgG2b isotype control (left panel).
  • C57Bl/6 bone marrow cells were stained with PerCP-Cy5.5 Anti-Mouse CD11b (65-0112) and 0.5 ug violetFluor™ 450 Anti-Mouse F4/80 Antigen (75-4801) (right panel) or 0.5 ug violetFluor™ 450 Rat IgG2b isotype control (left panel).

violetFluor™ 450 Anti-Mouse F4/80 Antigen (BM8.1)

Cat No. Size Price Quantity
75-4801-U025 25 µg $100.00
75-4801-U100 100 µg $226.00

Description

The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.

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The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.

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Product Details

Name violetFluor™ 450 Anti-Mouse F4/80 Antigen (BM8.1)
Cat. No. 75-4801
Alternative Names EMR1, Ly71
Gene ID 13733
Clone BM8.1
Isotype Rat IgG2b, κ
Reactivity Mouse
Cross Reactivity
Format violetFluor™ 450
Application Flow Cytometry
Citations*

Ioannou M, Alissafi T, Boon L, Boumpas D, and Verginis P. 2013. J. Immunol. 190: 2631-2640. (Flow Cytometry)

Papadopoulos G, Weinberg EO, Massari P, Gibson FC, Wetzler LM, Morgan EF, and Genco CA. 2013. J. Immunol. 190: 1148-1157.

Chen Q and Snapper CM. 2013. J. Immunol. 190: 1048-1055.

Rankin AL, Mumm JB, Murphy E, Turner S, Yu N, McClanahan TK, Bourne PA, Pierce RH, Kastelein R and Pflanz S. 2010. J. Immunol. 184(3): 1526-1535. (Immunohistochemistry - paraffin-embedded tissue)

Geutskens SB, Otonkoski T, Pulkkinen MA, Drexhage HA and Leenen PJ. 2005. J. Leukoc. Biol. 78(4): 845-52 (Immunohistochemistry - frozen tissue)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.