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C57Bl/6 splenocytes were stained with 0.25 ug violetFluor™  450 Anti-Mouse C8a (75-1886) (solid line) or 0.25 ug violetFluor™  450 Rat IgG2b isotype control (dashed line).
  • C57Bl/6 splenocytes were stained with 0.25 ug violetFluor™  450 Anti-Mouse C8a (75-1886) (solid line) or 0.25 ug violetFluor™  450 Rat IgG2b isotype control (dashed line).

violetFluor™ 450 Anti-Mouse CD8a (2.43)

Cat No. Size Price Quantity
75-1886-U025 25 µg $62.00
75-1886-U100 100 µg $142.00

Description

The 2.43 antibody reacts with the 32-34 kDa alpha subunit of mouse CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor in antigen recognition and subsequent T cell activation induced by binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck and facilitate intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death in tumor cells, as well as in cells infected by virus, bacteria or parasites.

The 2.43 antibody is widely used as a phenotypic marker for mouse CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.

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The 2.43 antibody reacts with the 32-34 kDa alpha subunit of mouse CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor in antigen recognition and subsequent T cell activation induced by binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck and facilitate intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death in tumor cells, as well as in cells infected by virus, bacteria or parasites.

The 2.43 antibody is widely used as a phenotypic marker for mouse CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.

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Product Details

Name violetFluor™ 450 Anti-Mouse CD8a (2.43)
Cat. No. 75-1886
Alternative Names CD8 alpha, Ly-2, Ly-35, Ly-B, Lyt-2
Gene ID 12525
Clone 2.43
Isotype Rat IgG2b
Reactivity Mouse
Cross Reactivity
Format violetFluor™ 450
Application Flow Cytometry
Citations*

Toriyama K, Kuwahara M, Kondoh H, et al. T cell-specific deletion of Pgam1 reveals a critical role for glycolysis in T cell responses. Commun Biol. 2020;3(1):394. Published 2020 Jul 24. doi:10.1038/s42003-020-01122-w.

» View on Communications Biology

Lin J-S, Szaba FM, Kummer LW, Chromy BA, and Smiley ST. 2011. J. Immunol. 187: 897-904. (in vivo depletion)

Wozniak KL, Young ML, and Wormley FL. 2011. Clin. Vaccine Immunol. 18(5):717-723. (in vivo depletion)

Hufford MM, Kim TS, Sun J, and Braciale TJ. 2011. J. Exp. Med. 208: 167-180 (in vivo depletion)

Ou R, zhang M, Huang L, Flavell RA, Koni PA, and Moskophidis D. 2008. J. Virol. 82:2952-2965. (Immunohistochemistry – OCT embedded frozen tissue)

Bosselut R, Zhang W, Ashe JM, Kopacz JL, Samelson LE, and Singer A. 1999. J. Exp. Med. 190: 1517-1526. (in vitro activation)

Davies A, Kalb, S, Liang B, Aldrich CJ, Lemonnier FA, Jiang H, Cotter R, and Soloski MJ. 2003. J. Immunol. 170: 5027-5033. (Blocking)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.