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Human peripheral blood monocytes were stained with 5 uL (0.5 ug) violetFluor™ 450 Anti-Human CD11c (75-0116) (solid line) or 0.5 ug violetFluor™ 450 Mouse IgG1 isotype control (dashed line).
  • Human peripheral blood monocytes were stained with 5 uL (0.5 ug) violetFluor™ 450 Anti-Human CD11c (75-0116) (solid line) or 0.5 ug violetFluor™ 450 Mouse IgG1 isotype control (dashed line).

violetFluor™ 450 Anti-Human CD11c (3.9)

Cat No. Size Price Quantity
75-0116-T025 25 tests $73.00
75-0116-T100 100 tests $167.00

Description

The 3.9 antibody reacts with human CD11c, also known as integrin αalpha X. This 150 kDa cell surface glycoprotein is part of a family of integrin αreceptors that mediate adhesion between ≥ ≥ ≥ cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrin αs are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin αreceptors consist of distinct combinations of integrin αchains which are differentially expressed. integrin αalpha X (CD11c) assembles with integrin αbeta-2 (CD18) into a receptor known as CR4 which can bind and induce signaling through ICAMs and VCAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.

The 3.9 antibody is widely used as a marker for CD11c expression on dendritic cells (DC), often in parallel with markers for CD11b, for identification of developmental stages and mature subsets of this cell type. CD11c is prominently expressed on tissue macrophages, and is also detected on activated neutrophils, granulocytes, some types of activated T cells and intestinal intraepithelial lymphocytes (IEL). The antibody is reported to be cross-reactive with Baboon, Chimpanzee, Cynomolgus and Rhesus CD11c.

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The 3.9 antibody reacts with human CD11c, also known as integrin αalpha X. This 150 kDa cell surface glycoprotein is part of a family of integrin αreceptors that mediate adhesion between ≥ ≥ ≥ cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrin αs are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin αreceptors consist of distinct combinations of integrin αchains which are differentially expressed. integrin αalpha X (CD11c) assembles with integrin αbeta-2 (CD18) into a receptor known as CR4 which can bind and induce signaling through ICAMs and VCAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.

The 3.9 antibody is widely used as a marker for CD11c expression on dendritic cells (DC), often in parallel with markers for CD11b, for identification of developmental stages and mature subsets of this cell type. CD11c is prominently expressed on tissue macrophages, and is also detected on activated neutrophils, granulocytes, some types of activated T cells and intestinal intraepithelial lymphocytes (IEL). The antibody is reported to be cross-reactive with Baboon, Chimpanzee, Cynomolgus and Rhesus CD11c.

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Product Details

Name violetFluor™ 450 Anti-Human CD11c (3.9)
Cat. No. 75-0116
Alternative Names CR4, integrin α_X, ITGAX
Gene ID 3687
Clone 3.9
Isotype Mouse IgG1, kappa
Reactivity Human
Cross Reactivity Baboon, Chimpanzee, Cynomolgus, Rhesus
Format violetFluor™ 450
Application Flow Cytometry
Citations*

Robinson BA, Estep RD, Messaoudi I, Rogers KS, and Wong SW. J. Virol. 2012. 86:2197-2211. (Flow cytometry – rhesus macaque)

Campillo-Gimenez L, Laforge M, Fay M, Brussel A, et. al. 2010. J. Virol. 84(4):1838-1846. (Flow cytometry – African green monkey, rhesus macaque)

Sadhu C, Hendrickson L, Dick KO, Potter TG, and Staunton DE. 2008. J. Immunoassay Immunochem. 29(1):42-57. (in vitro blocking)

Arndt S, Melle C, Mondal K, Klein G, von Eggeling F, and Bosserhoff A-K. 2007. J. Leukoc. Biol. 82:1466-1472. (Immunoprecipitation)

McGreal EP, Ikewaki N, Akatsu H, Morgan BP, and Gasque P. 2002. 168:5222-5232. (Immunofluorescence microscopy – frozen tissue)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.