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Recombinant Mouse VEGF-165 (Carrier-free)
  • Recombinant Mouse VEGF-165 (Carrier-free)

Recombinant Mouse VEGF-165 (Carrier-free)

Cat No. Size Price Quantity
21-8351-U002 2 µg $69.00
21-8351-U010 10 µg $195.00
21-8351-U100 100 µg $659.00
21-8351-U500 500 µg $1,691.00

Description

VEGFs are a family of molecules that are key drivers of vascularization and angiogenic processes. VEGFs induce proliferation and homeostasis of endothelial cells. VEGFs have been studied as indicators of both normal and pathogenic angiogenesis particularly involving tumor cells. Overexpression of VEGF in tumor cells allows tumor growth and enhanced metastatic potential. VEGF family members stimulate cellular responses by binding to VEGF receptors that include fms-like tyrosine kinase (flt-1), KDR gene product (the Mouse homolog of KDR is the flk-1 gene product), and the flt4 gene product. Splicing of the exon 6 or exon 7 mRNA from the 8 exon VEGF gene results in the different amino acid number and variants in humans and mice accounting for VEGF121, VEGF165 and others (one amino acid less in Mouse analogs). These variants differ in their binding to heparin sulfate proteoglycans and neuropilin co-receptors imparting differential binding and activation through VEGFRs and variant specific angiogenic or anti-angiogenic functions. VEGFs and VEGFRs are targets for therapeutic intervention.
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VEGFs are a family of molecules that are key drivers of vascularization and angiogenic processes. VEGFs induce proliferation and homeostasis of endothelial cells. VEGFs have been studied as indicators of both normal and pathogenic angiogenesis particularly involving tumor cells. Overexpression of VEGF in tumor cells allows tumor growth and enhanced metastatic potential. VEGF family members stimulate cellular responses by binding to VEGF receptors that include fms-like tyrosine kinase (flt-1), KDR gene product (the Mouse homolog of KDR is the flk-1 gene product), and the flt4 gene product. Splicing of the exon 6 or exon 7 mRNA from the 8 exon VEGF gene results in the different amino acid number and variants in humans and mice accounting for VEGF121, VEGF165 and others (one amino acid less in Mouse analogs). These variants differ in their binding to heparin sulfate proteoglycans and neuropilin co-receptors imparting differential binding and activation through VEGFRs and variant specific angiogenic or anti-angiogenic functions. VEGFs and VEGFRs are targets for therapeutic intervention.
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Product Details

Name Recombinant Mouse VEGF-165 (Carrier-free)
Cat. No. 21-8351
Alternative Names Vascular Endothelial Growth Factor, VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen, VEGF
Amino Acid Sequence MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-5.0 ng/ml.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Mouse VEGF-165 is a 39.0 kDa protein composed of two 165 amino acid residues.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source E. coli
Reactivity Mouse
Research Areas Angiogenesis/Cardiovascular; Cancer; Inflammation; Proliferation
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.