Recombinant Mouse Stem Cell Factor (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-8341-U002 | 2 µg | $61.00 | ||
| 21-8341-U010 | 10 µg | $175.00 |
Technical Data Sheet
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SCF, or c-kit ligand or steel factor, is a hematopoietic growth factor that exerts its activity by signaling through CD117, the c-Kit receptor. Soluble and transmembrane SCF is produced by a variety of cells including fibroblasts and endothelial cells and is critical for driving HSC survival, proliferation and differentiation. SCF potentially effects stem cell niche or microenvironment of stem cell location affecting HSC maintenance and along with SDF-1, allows stem cells to relocate into the niche. SCF plays a role in hematopoiesis, spermatogenesis, melanocyte development and other developmental activities. In vitro SCF and c-Kit also increase megakaryocyte development. SCF has clinical value for bone marrow transplantation but also enhances allergic reactions due to mast cell expansion through the CD117 c-kit receptor.
SCF, or c-kit ligand or steel factor, is a hematopoietic growth factor that exerts its activity by signaling through CD117, the c-Kit receptor. Soluble and transmembrane SCF is produced by a variety of cells including fibroblasts and endothelial cells and is critical for driving HSC survival, proliferation and differentiation. SCF potentially effects stem cell niche or microenvironment of stem cell location affecting HSC maintenance and along with SDF-1, allows stem cells to relocate into the niche. SCF plays a role in hematopoiesis, spermatogenesis, melanocyte development and other developmental activities. In vitro SCF and c-Kit also increase megakaryocyte development. SCF has clinical value for bone marrow transplantation but also enhances allergic reactions due to mast cell expansion through the CD117 c-kit receptor.
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Product Details
| Name | Recombinant Mouse Stem Cell Factor (SCF) (Carrier-free) |
|---|---|
| Cat. No. | 21-8341 |
| Alternative Names | c-kit ligand, steel factor |
| Amino Acid Sequence | MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | ED50 is < 10 ng/ml, corresponding to a specific activity of ≥≥>1 x 105 units/mg calculated by stimulation of the proliferation of the human TF-1 cells. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant Mouse SCF is an 18.3 kDa polypeptide containing 164 amino acid residues, identical to the sequence of secreted soluble form of SCF. |
| Protein Content | Content Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 98 |
| Source | E. coli |
| Reactivity | Mouse |
| Research Areas | Cell Culture, Proliferation, Stem Cells and Differentiation |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.