Recombinant Mouse Sonic Hedgehog (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-8671-U005 | 5 µg | $76.00 | ||
| 21-8671-U025 | 25 µg | $210.00 |
Technical Data Sheet
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Sonic Hedgehog (Shh) is one of three highly conserved proteins that share the same signaling pathway and make up the Hedgehog family. Sonic Hedgehog is expressed in key embryonic tissues, is involved in regulating the patterning of the developing nervous system, somite and limb, and also plays an important role in the development of certain tissues like hair, tooth and bone. It interacts with the Patched and Smoothened transmembrane receptors.
Sonic Hedgehog (Shh) is one of three highly conserved proteins that share the same signaling pathway and make up the Hedgehog family. Sonic Hedgehog is expressed in key embryonic tissues, is involved in regulating the patterning of the developing nervous system, somite and limb, and also plays an important role in the development of certain tissues like hair, tooth and bone. It interacts with the Patched and Smoothened transmembrane receptors.
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Product Details
| Name | Recombinant Mouse Sonic Hedgehog (Carrier-free) |
|---|---|
| Cat. No. | 21-8671 |
| Alternative Names | Shh, Hhg-1 |
| Amino Acid Sequence | IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.5-1.0 µg/ml. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant Mouse Sonic Hedgehog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the naturally occurring, chemically modified Cys residue. |
| Protein Content | Content Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 90 |
| Source | E. coli |
| Reactivity | Mouse |
| Research Areas | Apoptosis; Bone, Skeletal, Cartilage; Immune System; Stem Cells & Differentiation; Neurobiology |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.