Recombinant Mouse sCD40 Ligand (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-8512-U005 | 5 µg | $69.00 | ||
| 21-8512-U025 | 25 µg | $199.00 | ||
| 21-8512-U100 | 100 µg | $625.00 | ||
| 21-8512-U500 | 500 µg | $1,795.00 |
Technical Data Sheet
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CD40 ligand, also known as CD154, is a cell surface glycoprotein expressed on the surface of activated T cells. Binding of CD40 ligand to CD40, expressed on B cells, stimulates B cell proliferation and secretion of all immunoglobulin isotypes.
CD40 ligand, also known as CD154, is a cell surface glycoprotein expressed on the surface of activated T cells. Binding of CD40 ligand to CD40, expressed on B cells, stimulates B cell proliferation and secretion of all immunoglobulin isotypes.
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Product Details
| Name | Recombinant Mouse sCD40 Ligand (Carrier-free) |
|---|---|
| Cat. No. | 21-8512 |
| Alternative Names | soluble CD40 Ligand, TNF-αSF5, TRAP, CD154, gp39, T-BAM |
| Amino Acid Sequence | MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | Determined by its ability to induce TNF-alpha and MIP-1 alpha production by mouse splenocytes. The expected ED50 for this effect is < 0.1 µg/ml. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant Mouse CD40L is a soluble 16.4 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-αlike domain of CD40L. |
| Protein Content | Content Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 98 |
| Source | E. coli |
| Reactivity | Mouse |
| Research Areas | Angiogenesis/Cardiovascular; Apoptosis; Immune System; Inflammation; Proliferation; TNF-α Superfamily; Allergy; Transplantation |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.