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Recombinant Mouse IL-12 p70 (Carrier-free)
  • Recombinant Mouse IL-12 p70 (Carrier-free)

Recombinant Mouse IL-12 p70 (Carrier-free)

Cat No. Size Price Quantity
21-8121-U002 2 µg $72.00
21-8121-U010 10 µg $195.00
21-8121-U100 100 µg $1,000.00

Description

Interleukin-12 (IL-12) is a heterodimeric pro-inflammatory cytokine composed of IL-12A (p35) and IL-12B (p40) acting on predominantly T cells and NK cells. IL-12 is a strong inflammatory mediator produced by dendritic cells (DCs) and phagocytes upon exposure to pathogens also indicating its bridging of innate and adaptive immune responses. IL-12 appears to be important in T cell (CD4 and CD8) maturation and NK cell activity. IL-12 induces IFN-gamma and TNF-alpha from both NK cells and T cells while diminishing IL-4 mediated suppression of IFN-gamma.
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Interleukin-12 (IL-12) is a heterodimeric pro-inflammatory cytokine composed of IL-12A (p35) and IL-12B (p40) acting on predominantly T cells and NK cells. IL-12 is a strong inflammatory mediator produced by dendritic cells (DCs) and phagocytes upon exposure to pathogens also indicating its bridging of innate and adaptive immune responses. IL-12 appears to be important in T cell (CD4 and CD8) maturation and NK cell activity. IL-12 induces IFN-gamma and TNF-alpha from both NK cells and T cells while diminishing IL-4 mediated suppression of IFN-gamma.
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Product Details

Name Recombinant Mouse IL-12 p70 (Carrier-free)
Cat. No. 21-8121
Alternative Names NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF
Amino Acid Sequence

p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA

p40 Subu

Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity The ED50 was determined by the stimulation of IFN-gamma production by murine splenocytes co-stimulated with IL-18 is < 0.1 ng/ml, corresponding to a specific activity of ≥≥>1 x 107 units/mg.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Mouse IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (506 total amino acid residues).
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source CHO cells
Reactivity Mouse
Research Areas Angiogenesis/Cardiovascular; Cancer; Immune System; Inflammation; Stem Cells & Differentiation; Allergy; Transplantation
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.