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Recombinant Mouse IL-10 (Carrier-free)
  • Recombinant Mouse IL-10 (Carrier-free)

Recombinant Mouse IL-10 (Carrier-free)

Cat No. Size Price Quantity
21-8101-U002 2 µg $61.00
21-8101-U010 10 µg $175.00
21-8101-U050 50 µg $535.00
21-8101-U500 500 µg $2,210.00

Description

IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells, mast cells in mice, and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF-alpha. Similar to IL-4, IL-10 enhances humoral immune responses through Th2 cells and B cells. IL-10 attenuates cell-mediated immune reactions. IL-10 inhibits synthesis of pro-inflammatory cytokines from macrophages and Tregs and also suppresses the antigen-presentation capacity of antigen presenting cells. IL-10 is stimulatory for Th2 cells and enhances B cell maturation and antibody production.
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IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells, mast cells in mice, and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF-alpha. Similar to IL-4, IL-10 enhances humoral immune responses through Th2 cells and B cells. IL-10 attenuates cell-mediated immune reactions. IL-10 inhibits synthesis of pro-inflammatory cytokines from macrophages and Tregs and also suppresses the antigen-presentation capacity of antigen presenting cells. IL-10 is stimulatory for Th2 cells and enhances B cell maturation and antibody production.
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Product Details

Name Recombinant Mouse IL-10 (Carrier-free)
Cat. No. 21-8101
Alternative Names B-TCGF, CSIF, TGIF
Amino Acid Sequence MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGQKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLEDQGVYKA MNEFDIFINC IEAYMMIKMK S
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Measured by the dose-dependent co-stimulation (with IL-4) of the proliferation of murine MC/9 cells. The ED50 was found to be < 2 ng/ml, corresponding to a specific activity of ≥≥>5 x 105 units/mg.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Mouse IL-10 is an 18.7 kDa protein of 161 amino acid residues.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source E. coli
Reactivity Mouse
Research Areas AIDS/HIV; Cancer; Immune System; Inflammation;Neurobiology; Stem Cells & Differentiation; Allergy; Transplantation
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.