Recombinant Mouse HGF (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-8431-U005 | 5 µg | $76.00 | ||
| 21-8431-U020 | 20 µg | $199.00 | ||
| 21-8431-U100 | 100 µg | $769.00 | ||
| 21-8431-U500 | 500 µg | $1,985.00 |
Technical Data Sheet
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Hepatocyte Growth Factor (HGF), also known as Hepatopoietin A and Scatter Factor, is a dimeric molecule composed of the ?-subunit and beta-subunit, linked by a disulfide bond. It is secreted as a single inactive polypeptide and this pro-petide is cleaved by serine proteases into alpha- and beta-chains. HGF is reported to be a hepatrophic factor and acts as a growth factor for a wide variety of tissues and cell types. Its functions include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. The protein is secreted by mesenchymal cells and, after binding to the proto-oncogenic c-Met receptor, activates a tyrosine kinase signaling cascade. Hypoxia and hyperglycemia suppress HGF production.
Hepatocyte Growth Factor (HGF), also known as Hepatopoietin A and Scatter Factor, is a dimeric molecule composed of the ?-subunit and beta-subunit, linked by a disulfide bond. It is secreted as a single inactive polypeptide and this pro-petide is cleaved by serine proteases into alpha- and beta-chains. HGF is reported to be a hepatrophic factor and acts as a growth factor for a wide variety of tissues and cell types. Its functions include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. The protein is secreted by mesenchymal cells and, after binding to the proto-oncogenic c-Met receptor, activates a tyrosine kinase signaling cascade. Hypoxia and hyperglycemia suppress HGF production.
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Product Details
| Name | Recombinant Mouse HGF (Carrier-free) |
|---|---|
| Cat. No. | 21-8431 |
| Alternative Names | Scatter Factor (SF), Hepatopoietin (HPTA) |
| Amino Acid Sequence | Alpha chain: QKKRRNTLHE FKKSAKTTLT KEDPLLKIKT KKVNSADECA NRCIRNRGFT FTCKAFVFDK SRKRCYWYPF NSMSSGVKKG FGHEFDLYEN KDYIRNCIIG KGGSYKGTVS ITKSGIKCQP WNSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG PMDHTESGKT CQRWDQQTPH RHKF |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | Determined by the dose-dependent stimulation of the proliferation of mouse IMCD3 cells using a concentration range of 10-20 ng/ml. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant mouse HGF, under reducing conditions, has a 69-kDa alpha-chain and 34-kDa beta-chain. The alpha-chain consists of 463 amino acid residues and the beta-chain consists of ≥ 234 amino acid residues. |
| Protein Content | Content Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 95 |
| Source | (BTI-Tn-5B1-4) Hi-5 Insect cells* |
| Reactivity | Mouse |
| Research Areas | Angiogenesis/Cardiovascular; Cancer; Neurobiology; Proliferation; Stem Cells & Differentiation; Wound Healing |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.