Recombinant Mouse EGF (Carrier-free)
  • Recombinant Mouse EGF (Carrier-free)

Recombinant Mouse EGF (Carrier-free)

Cat No. Size Price Quantity
21-8357-U100 100 µg $75.00
21-8357-U500 500 µg $185.00
21-8357-M001 1 mg $289.00
Lead time: 2-3 business days

Description

Epidermal growth factor, EGF, through binding to its receptor EGFR stimulates cell growth, proliferation and differentiation. EGF can be found in many cells including mammary gland cells, macrophages, gut epithelial cells as well as in the nervous system and kidney. As a potent mitogen, EGF can induce proliferation of several tissue types in cell culture.
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Epidermal growth factor, EGF, through binding to its receptor EGFR stimulates cell growth, proliferation and differentiation. EGF can be found in many cells including mammary gland cells, macrophages, gut epithelial cells as well as in the nervous system and kidney. As a potent mitogen, EGF can induce proliferation of several tissue types in cell culture.
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Product Details

Name Recombinant Mouse EGF (Carrier-free)
Cat. No. 21-8357
Alternative Names Epidermal Growth Factor, Urogastrone, URG
Amino Acid Sequence NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity The ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1x 107 units/mg.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Mouse EGF is a 6.0 kDa globular protein composed of ≥ 53 amino acid residues.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source E. coli
Reactivity Mouse
Research Areas Angiogenesis/Cardiovascular; Cancer; Cell Culture?;? Inflammation ; Neurobiology; Proliferation; Stem Cells & Differentiation; Wound Healing
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.