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Recombinant Human WISP-3 (Carrier-Free)
  • Recombinant Human WISP-3 (Carrier-Free)

Recombinant Human WISP-3 (Carrier-Free)

Cat No. Size Price Quantity
21-9075-U005 5 µg $80.00
21-9075-U020 20 µg $195.00
21-9075-U100 100 µg $780.00
21-9075-U500 500 µg $2,340.00

Description

WISP-3 is a member of the CCN family of secreted cysteine rich regulatory proteins. It is predominantly expressed in the adult kidney, testis, and fetal kidney, but it is also found with weaker expression in the placenta, ovary, prostate, small intestine, and skeletally-derived cells. It is composed of four distinct structural domains (modules): the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the thrombospondin type-1 repeat (TSP type-1) domain, and a C-terminal cysteine knot-like (CTCK) domain.
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WISP-3 is a member of the CCN family of secreted cysteine rich regulatory proteins. It is predominantly expressed in the adult kidney, testis, and fetal kidney, but it is also found with weaker expression in the placenta, ovary, prostate, small intestine, and skeletally-derived cells. It is composed of four distinct structural domains (modules): the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the thrombospondin type-1 repeat (TSP type-1) domain, and a C-terminal cysteine knot-like (CTCK) domain.
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Product Details

Name Recombinant Human WISP-3 (Carrier-Free)
Cat. No. 21-9075
Alternative Names WNT-1 inducible signaling pathway protein-3, CCN6
Amino Acid Sequence TGPLDTTPEG RPGEVSDAPQ RKQFCHWPCK CPQQKPRCPP GVSLVRDGCG CCKICAKQPG EICNEADLCD PHKGLYCDYS VDRPRYETGV CAYLVAVGCE FNQVHYHNGQ VFQPNPLFSC LCVSGAIGCT PLFIPKLAGS HCSGAKGGKK SDQSNCSLEP LLQQLSTSYK TMPAYRNLPL IWKKKCLVQA TKWTPCSRTC GMGISNRVTN ENSNCEMRKE KRLCYIQPCD SN
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity The ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 0.2-0.3 µg/ml.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human WISP-3 is a 36.8 kDa protein containing 331 amino acid residues.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source E.coli
Reactivity Human
Research Areas Angiogenesis/Cardiovascular, Apoptosis
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.