Recombinant Human Visfatin (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-7092-U005 | 5 µg | $76.00 | ||
| 21-7092-U025 | 25 µg | $175.00 | ||
| 21-7092-U100 | 100 µg | $585.00 | ||
| 21-7092-U500 | 500 µg | $1,795.00 |
Technical Data Sheet
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Visfatin, also known as pre-B cell colony enhancing factor (PBEF) is an adipocytokine belonging to the NAPRTase family and is produced and secreted primarily by white adipose tissue. Like insulin, Visfatin exerts hypoglycemic effects by interacting with the insulin receptor. Visfatin does not, however, compete with insulin for binding to this shared receptor and circulating levels of visfatin are much lower than that of insulin. Furthermore, the levels of visfatin are not influenced by food intake. The plasma Visfatin levels, like those of Leptin, correlate positively with the percent of body fat and increase during the development of obesity. The expression of Visfatin is upregulated by hypoxia, inflammation and hyperglycemia and downregulated by insulin, somatostatin and statins.
Visfatin, also known as pre-B cell colony enhancing factor (PBEF) is an adipocytokine belonging to the NAPRTase family and is produced and secreted primarily by white adipose tissue. Like insulin, Visfatin exerts hypoglycemic effects by interacting with the insulin receptor. Visfatin does not, however, compete with insulin for binding to this shared receptor and circulating levels of visfatin are much lower than that of insulin. Furthermore, the levels of visfatin are not influenced by food intake. The plasma Visfatin levels, like those of Leptin, correlate positively with the percent of body fat and increase during the development of obesity. The expression of Visfatin is upregulated by hypoxia, inflammation and hyperglycemia and downregulated by insulin, somatostatin and statins.
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Product Details
| Name | Recombinant Human Visfatin (Carrier-free) |
|---|---|
| Cat. No. | 21-7092 |
| Alternative Names | PBEF (Pre-B cell colony-enhancing factor) |
| Amino Acid Sequence | MPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DH |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | The ED50was determined by the dose-dependant proliferation of the RPMI 8226 cells. The expected ED50for this effect is 15.0-20.0 ng/ml. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant human Visfatin is a 52.6 kDa protein containing 466 amino acid residues (isoform 1). |
| Protein Content | Content Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 98 |
| Source | E. coli |
| Reactivity | Human |
| Research Areas | Diabetes / Weight Regulation; Lipid Metabolism |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.