Recombinant Human TWEAK (CD255) (Carrier-free)
|Name||Recombinant Human TWEAK (CD255) (Carrier-free)|
|Alternative Names||TNFRelated weak inducer of apoptosis, TNF-αSF12, DR3LG, Apo3-Ligand|
|Amino Acid Sequence||MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH|
|Authenticity||Verified by N-terminal and Mass Spectrometry analyses (when applicable).|
Assay #1: The ED50 as determined by the dose-dependent stimulation of IL-8 production by Human PBMC is less than 10 ng/ml.
Assay #2: TWEAK weakly induces the death of HT29 cells when cul
|Endotoxin Level||Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).|
|Molecular Mass||Recombinant human TWEAK is a soluble 17.0 kDa polypeptide (154 amino acid residues) comprising the TNF-α homologous region of TWEAK and is generated by proteolytic processing of the full length membrane anchored TWEAK protein.|
|Protein Content||Content Verified by UV Spectroscopy and/or SDS-PAGE gel.|
|Research Areas||Angiogenesis/Cardiovascular; Apoptosis; Inflammation; Neurobiology; Proliferation; TNF-α Superfamily|
|Reconstitution||See Certificate of Analysis (COA) for lot specific reconstitution information.|
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.