Recombinant Human TSG (Carrier-Free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-9066-U010 | 10 µg | $80.00 | ||
| 21-9066-U050 | 50 µg | $195.00 | ||
| 21-9066-U100 | 100 µg | $300.00 | ||
| 21-9066-U500 | 500 µg | $900.00 |
Technical Data Sheet
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Twisted Gastrulation Protein (TSG) is a secreted BMP-binding protein that is structurally related to the BMP antagonists Chordin and Noggin. TSG can inhibit BMP activity by binding directly to BMP proteins, and can act either as a BMP-4 agonist or antagonist (depending on the specific biochemical environment) by binding to the BMP-4/Chordin complex.
Twisted Gastrulation Protein (TSG) is a secreted BMP-binding protein that is structurally related to the BMP antagonists Chordin and Noggin. TSG can inhibit BMP activity by binding directly to BMP proteins, and can act either as a BMP-4 agonist or antagonist (depending on the specific biochemical environment) by binding to the BMP-4/Chordin complex.
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Product Details
| Name | Recombinant Human TSG (Carrier-Free) |
|---|---|
| Cat. No. | 21-9066 |
| Alternative Names | Twisted Gastrulation Protein |
| Amino Acid Sequence | CNKALCASDV SKCLIQELCQ CRPGEGNCSC CKECMLCLGA LWDECCDCVG MCNPRNYSDT PPTSKSTVEE LHEPIPSLFR ALTEGDTQLN WNIVSFPVAE ELSHHENLVS FLETVNQPHH QNVSVPSNNV HAPYSSDKEH MCTVVYFDDC MSIHQCKISC ESMGASKYRW FHNACCECIG PECIDYGSKT VKCMNCMF |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | Determined by its ability to neutralize BMP-6-induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is ≥ 2.0 ug/ml of TSG. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant Human TSG is a 198 amino acid, 22.2 kDa protein containing the BMP/TGF-beta binding portion of the full length TSG protein. |
| Protein Content | Content Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 98 |
| Source | E.coli |
| Reactivity | Human |
| Research Areas | Bones, Skeletal, Cartilage |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.