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Recombinant Human TNF-a (Carrier-free)
  • Recombinant Human TNF-a (Carrier-free)

Recombinant Human TNF-a (Carrier-free)

Cat No. Size Price Quantity
21-8329-U010 10 µg $125.00
21-8329-U050 50 µg $195.00
21-8329-U100 100 µg $342.00
21-8329-U500 500 µg $798.00

Description

TNF-alpha is a clinically important cytokine with strong pro-inflammatory activities. TNF-alpha is secreted by a variety of immune and non-immune cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. TNF-alpha signals through two receptors, TNFR1 and TNFR2. TNF-alpha has strong cytolytic activities against a wide variety of tumor cells and is critically important in combat against bacterial infections. The inflammatory effects of TNF-alpha are also deleterious in causation of septic shock, and autoimmune diseases including rheumatoid arthritis, inflammation, and diabetes.
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TNF-alpha is a clinically important cytokine with strong pro-inflammatory activities. TNF-alpha is secreted by a variety of immune and non-immune cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. TNF-alpha signals through two receptors, TNFR1 and TNFR2. TNF-alpha has strong cytolytic activities against a wide variety of tumor cells and is critically important in combat against bacterial infections. The inflammatory effects of TNF-alpha are also deleterious in causation of septic shock, and autoimmune diseases including rheumatoid arthritis, inflammation, and diabetes.
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Product Details

Name Recombinant Human TNF-αα (Carrier-free)
Cat. No. 21-8329
Alternative Names Tumor Necrosis Factor, TNF-αSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin
Amino Acid Sequence VRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity ED50 is determined by the cytolysis of Mouse L929 cells in the presence of Actinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human TNF-α alpha is a soluble 157 amino acid protein of 17.4 kDa which corresponds to C-terminal extracellular domain of the full length transmembrane protein.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source E. coli
Reactivity Human
Research Areas AIDS/HIV; Angiogenesis/Cardiovascular; Apoptosis; Cancer; Diabetes / Weight Regulation; Immune System; Inflammation; Neurobiology; Stem Cells & Differentiation; TNF-α Superfamily; Allergy; Transplantation
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.