

Recombinant Human TNF-a (Carrier-free)
Cat No. | Size | Price | Quantity | |
---|---|---|---|---|
21-8329-U010 | 10 µg | $125.00 | ||
21-8329-U050 | 50 µg | $195.00 | ||
21-8329-U100 | 100 µg | $342.00 | ||
21-8329-U500 | 500 µg | $798.00 |
Description
Product Details
Name | Recombinant Human TNF-αα (Carrier-free) |
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Cat. No. | 21-8329 |
Alternative Names | Tumor Necrosis Factor, TNF-αSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin |
Amino Acid Sequence | VRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL |
Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
Bioactivity | ED50 is determined by the cytolysis of Mouse L929 cells in the presence of Actinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg. |
Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
Molecular Mass | Recombinant Human TNF-α alpha is a soluble 157 amino acid protein of 17.4 kDa which corresponds to C-terminal extracellular domain of the full length transmembrane protein. |
Protein Content | Content Verified by UV Spectroscopy and/or SDS-PAGE gel. |
Purity (%) | 98 |
Source | E. coli |
Reactivity | Human |
Research Areas | AIDS/HIV; Angiogenesis/Cardiovascular; Apoptosis; Cancer; Diabetes / Weight Regulation; Immune System; Inflammation; Neurobiology; Stem Cells & Differentiation; TNF-α Superfamily; Allergy; Transplantation |
Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
Application | Bioassay |
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.