Recombinant Human TLR-4 (Carrier-free)
  • Recombinant Human TLR-4 (Carrier-free)

Recombinant Human TLR-4 (Carrier-free)

Cat No. Size Price Quantity
21-9232-U010 10 µg $70.00
21-9232-U050 50 µg $180.00
21-9232-U100 100 µg $290.00
21-9232-U250 250 µg $490.00
21-9232-U500 500 µg $850.00
21-9232-M001 1 mg $1,450.00
Lead time: 2-3 business days

Description

TLR4 is a 100 kDa type I transmembrane glycoprotein member of the toll-like receptor (TLR) family that plays a fundamental role in pathogen recognition and activation of innate immunity. Mature TLR4 protein contains 21 leucine rich repeats in its extracellular domain and one cytoplasmic Toll/IL‑1 receptor (TIR) domain. TLRs are pathogen pattern recognition molecules and TLR4 primarily functions as a receptor for bacterial endotoxin/lipopolysaccharide (LPS), the major cell wall component of gram-negative bacteria. TLR4 cooperates with LY96 (also referred as MD-2) and CD14 to mediate in downstream signal transduction events that promote tissue inflammation. Recombinant Human TLR-4 is a 69.3 kDa glycoprotein containing 609 amino acid residues of the TLR-4 extracellular domain. As a result of glycosylation, Recombinant Human TLR-4 migrates with an apparent molecular mass of approximately 90-100 kDa by SDS-PAGE gel, under reducing and non-reducing conditions.
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TLR4 is a 100 kDa type I transmembrane glycoprotein member of the toll-like receptor (TLR) family that plays a fundamental role in pathogen recognition and activation of innate immunity. Mature TLR4 protein contains 21 leucine rich repeats in its extracellular domain and one cytoplasmic Toll/IL‑1 receptor (TIR) domain. TLRs are pathogen pattern recognition molecules and TLR4 primarily functions as a receptor for bacterial endotoxin/lipopolysaccharide (LPS), the major cell wall component of gram-negative bacteria. TLR4 cooperates with LY96 (also referred as MD-2) and CD14 to mediate in downstream signal transduction events that promote tissue inflammation. Recombinant Human TLR-4 is a 69.3 kDa glycoprotein containing 609 amino acid residues of the TLR-4 extracellular domain. As a result of glycosylation, Recombinant Human TLR-4 migrates with an apparent molecular mass of approximately 90-100 kDa by SDS-PAGE gel, under reducing and non-reducing conditions.
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Product Details

Name Recombinant Human TLR-4 (Carrier-free)
Cat. No. 21-9232
Alternative Names Toll-like receptor 4, TOLL, CD284, ARMD10
Amino Acid Sequence ESWEPCVEVV PNITYQCMEL NFYKIPDNLP FSTKNLDLSF NPLRHLGSYS FFSFPELQVL DLSRCEIQTI EDGAYQSLSH LSTLILTGNP IQSLALGAFS GLSSLQKLVA VETNLASLEN FPIGHLKTLK ELNVAHNLIQ SFKLPEYFSN LTNLEHLDLS SNKIQSIYCT DLRVLHQMPL LNLSLDLSLN PMNFIQPGAF KEIRLHKLTL RNNFDSLNVM KTCIQGLAGL EVHRLVLGEF RNEGNLEKFD KSALEGLCNL TIEEFRLAYL DYYLDDIIDL FNCLTNVSSF SLVSVTIERV KDFSYNFGWQ HLELVNCKFG QFPTLKLKSL KRLTFTSNKG GNAFSEVDLP SLEFLDLSRN GLSFKGCCSQ SDFGTTSLKY LDLSFNGVIT MSSNFLGLEQ LEHLDFQHSN LKQMSEFSVF LSLRNLIYLD ISHTHTRVAF NGIFNGLSSL EVLKMAGNSF QENFLPDIFT ELRNLTFLDL SQCQLEQLSP TAFNSLSSLQ VLNMSHNNFF SLDTFPYKCL NSLQVLDYSL NHIMTSKKQE LQHFPSSLAF LNLTQNDFAC TCEHQSFLQW IKDQRQLLVE VERMECATPS DKQGMPVLSL NITCQMNKT
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Determined by its ability to bind rhMD-2 in a functional ELISA.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass 69.3 kDa
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 95
Source HEK293
Reactivity Human
Research Areas Angiogenesis/Cardiovascular, Allergy, Apoptosis, Cancer, Diabetes/Weight Regulation, Immune System, Inflammation, Proliferation, Receptors
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.