Recombinant Human TLR-3 (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-7087-U005 | 5 µg | $76.00 | ||
| 21-7087-U025 | 25 µg | $175.00 | ||
| 21-7087-U100 | 100 µg | $585.00 | ||
| 21-7087-U500 | 500 µg | $1,795.00 |
Technical Data Sheet
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Toll-Like Receptor-3 (TLR-3) is a member of the TLR family of pathogen pattern recognition molecules that play an important role in activation of the innate immune system. TLR-3 may be localized adjacent to the phagosomes of dendritic cells and macrophages, primarily in the placenta and pancreas. TLR-3 binds to and signals the presence of double stranded RNA (dsRNA), leading to activation of NF-kappaB and the production of type I interferons, initiating an antiviral immune response.
Toll-Like Receptor-3 (TLR-3) is a member of the TLR family of pathogen pattern recognition molecules that play an important role in activation of the innate immune system. TLR-3 may be localized adjacent to the phagosomes of dendritic cells and macrophages, primarily in the placenta and pancreas. TLR-3 binds to and signals the presence of double stranded RNA (dsRNA), leading to activation of NF-kappaB and the production of type I interferons, initiating an antiviral immune response.
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Product Details
| Name | Recombinant Human TLR-3 (Carrier-free) |
|---|---|
| Cat. No. | 21-7087 |
| Alternative Names | Toll-like receptor 3, CD283 antigen |
| Amino Acid Sequence | STTKCTVSHE VADCSHLKLT QVPDDLPTNI TVLNLTHNQL RRLPAANFTR YSQLTSLDVG FNTISKLEPE LCQKLPMLKV LNLQHNELSQ LSDKTFAFCT NLTELHLMSN SIQKIKNNPF VKQKNLITLD LSHNGLSSTK LGTQVQLENL QELLLSNNKI QALKSEELDI FANSSLKKLEL SSNQIKEFSP GCFHAIGRLF GLFLNNVQLG PSLTEKLCLE LANTSIRNLS L |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | Determined by its ability to inhibit IL-8 secretion induced by Poly I:C in the TLR3 receptor-expressing HEK293 cells (HEK293 TLR3r). The ED50for this effect is ≥ 30 µg/ml in the presence of 100 µg/ml of Poly I:C. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant human TLR-3 is 77.4 kDa glycoprotein containing 681 residues which comprise the TLR-3 extracellular domain. |
| Protein Content | Content Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 95 |
| Source | HEK293 cells |
| Reactivity | Human |
| Research Areas | Apoptosis; Inflammation; Receptors |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.