Recombinant Human Tissue Factor (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-9233-U002 | 2 µg | $70.00 | ||
| 21-9233-U010 | 10 µg | $180.00 | ||
| 21-9233-U050 | 50 µg | $600.00 | ||
| 21-9233-U100 | 100 µg | $950.00 | ||
| 21-9233-U250 | 250 µg | $1,750.00 | ||
| 21-9233-U500 | 500 µg | $3,050.00 | ||
| 21-9233-M001 | 1 mg | $4,900.00 |
Technical Data Sheet
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Tissue factor (TF), a single-pass type I membrane glycoprotein is also known as coagulation factor III, F3, and CD142. TF functions as a cofactor for plasma clotting protease factor VIIa (FVIIa) to initiate the coagulation cascade by activating clotting proteins factors IX and X. Tissue factor is stored on the surface of mononuclear and endothelial cells in microparticles that can shed into circulation in response to vascular injury, pro‐inflammatory cytokines, or microbial ligands. Recombinant Human Tissue Factor is a glycoprotein homodimer comprised of the 227‐amino‐acid length monomer containing a C‐terminal His‐Tag. It has a calculated molecular weight of approximately 25.8 kDa, and as a result of glycosylation, Recombinant Human Tissue Factor migrates with an apparent molecular mass of approximately 40‐50 kDa by SDS‐PAGE gel.
Tissue factor (TF), a single-pass type I membrane glycoprotein is also known as coagulation factor III, F3, and CD142. TF functions as a cofactor for plasma clotting protease factor VIIa (FVIIa) to initiate the coagulation cascade by activating clotting proteins factors IX and X. Tissue factor is stored on the surface of mononuclear and endothelial cells in microparticles that can shed into circulation in response to vascular injury, pro‐inflammatory cytokines, or microbial ligands. Recombinant Human Tissue Factor is a glycoprotein homodimer comprised of the 227‐amino‐acid length monomer containing a C‐terminal His‐Tag. It has a calculated molecular weight of approximately 25.8 kDa, and as a result of glycosylation, Recombinant Human Tissue Factor migrates with an apparent molecular mass of approximately 40‐50 kDa by SDS‐PAGE gel.
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Product Details
| Name | Recombinant Human Tissue Factor (Carrier-free) |
|---|---|
| Cat. No. | 21-9233 |
| Alternative Names | TF, TF1, Coagulation factor III, factor III, F3, Thromboplastin, CD142 |
| Amino Acid Sequence | SGTTNTVAAY NLTWKSTNFK TILEWEPKPV NQVYTVQIST KSGDWKSKCF YTTDTECDLT DEIVKDVKQT YLARVFSYPA GNVESTGSAG EPLYENSPEF TPYLETNLGQ PTIQSFEQVG TKVNVTVEDE RTLVRRNNTF LSLRDVFGKD LIYTLYYWKS SSSGKKTAKT NTNEFLIDVD KGENYCFSVQ AVIPSRTVNR KSTDSPVECM GGEKGEFREH HHHHHHH |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | Functional activity of Tissue Factor is determined by its ability to activate fluorogenic peptide substrate Boc-VPR-AMC cleavage, when bound in 1:1 complex with Coagulation Factor VII. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | 25.8 kDa |
| Protein Content | Content Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 98 |
| Source | CHO |
| Reactivity | Human |
| Research Areas | |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.