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Recombinant Human TGF-alpha (Carrier-free)
  • Recombinant Human TGF-alpha (Carrier-free)

Recombinant Human TGF-alpha (Carrier-free)

Cat No. Size Price Quantity
21-8698-U020 20 µg $76.00
21-8698-U100 100 µg $179.00
21-8698-U250 250 µg $355.00
21-8698-U500 500 µg $649.00

Description

Transforming Growth Factor alpha (TGF-alpha) is a member of the EGF subfamily, shares over 40% identity with EGF, and binds to the EGF receptor (EGFR) to stimulate the proliferation of a wide range of epidermal and epithelial cells. TGF-alpha also has angiogenic properties. Binding to the EGFR activates the PI3K and Erk1/2 pathways. It is expressed by keratinocytes, epithelial cells, macrophages and various tumors and tumor cell lines. Overexpression of TGF-alpha has been linked to a variety of cancers including breast, colon, and stomach cancers.
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Transforming Growth Factor alpha (TGF-alpha) is a member of the EGF subfamily, shares over 40% identity with EGF, and binds to the EGF receptor (EGFR) to stimulate the proliferation of a wide range of epidermal and epithelial cells. TGF-alpha also has angiogenic properties. Binding to the EGFR activates the PI3K and Erk1/2 pathways. It is expressed by keratinocytes, epithelial cells, macrophages and various tumors and tumor cell lines. Overexpression of TGF-alpha has been linked to a variety of cancers including breast, colon, and stomach cancers.
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Product Details

Name Recombinant Human TGF-alpha (Carrier-free)
Cat. No. 21-8698
Alternative Names Transforming Growth Factor-alpha, Sarcoma growth factor, TGF-type I, ETGF
Amino Acid Sequence VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 106 units/mg.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant human TGF-alpha is a 50 amino acid polypeptide (5.5 kDa) which shares approximately 40% sequence homology with EGF, including 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source E. coli
Reactivity Human
Research Areas Angiogenesis/Cardiovascular; Cancer; Inflammation; Wound Healing
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.