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Recombinant Human sTNF Receptor Type II (Carrier-free)
  • Recombinant Human sTNF Receptor Type II (Carrier-free)

Recombinant Human sTNF Receptor Type II (Carrier-free)

Cat No. Size Price Quantity
21-7084-U005 5 µg $76.00
21-7084-U020 20 µg $195.00
21-7084-U100 100 µg $555.00
21-7084-U500 500 µg $1,525.00

Description

TNF-α receptor II (TNF-αcεRII or TNFRSF1B), along with the structurally related TNFRI, belong to the TNF-α receptor superfamily of proteins. TNF-αcεRII has a more restricted expression pattern than TNFRI, limited mainly to hematopoietic and immune system cells, whereas TNFRI is thought to be present on all cell types. TNF-αcεRII is a high-affinity receptor for TNF-alpha and also binds TNF-αbeta, but at a lower affinity. Binding to membrane-bound TNF-α results in effective activation, but while soluble TNF-α binds to TNF-αcεRII, it does not activate its signaling pathway. Signaling through this receptor regulates various biological processes, including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. The soluble form of TNF-αcεRII is capable of inhibiting TNF-alpha-induced activities by acting as a decoy receptor, thereby reducing the inflammatory response.
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TNF-α receptor II (TNF-αcεRII or TNFRSF1B), along with the structurally related TNFRI, belong to the TNF-α receptor superfamily of proteins. TNF-αcεRII has a more restricted expression pattern than TNFRI, limited mainly to hematopoietic and immune system cells, whereas TNFRI is thought to be present on all cell types. TNF-αcεRII is a high-affinity receptor for TNF-alpha and also binds TNF-αbeta, but at a lower affinity. Binding to membrane-bound TNF-α results in effective activation, but while soluble TNF-α binds to TNF-αcεRII, it does not activate its signaling pathway. Signaling through this receptor regulates various biological processes, including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. The soluble form of TNF-αcεRII is capable of inhibiting TNF-alpha-induced activities by acting as a decoy receptor, thereby reducing the inflammatory response.
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Product Details

Name Recombinant Human sTNF-α Receptor Type II (Carrier-free)
Cat. No. 21-7084
Alternative Names Soluble Tumor Necrosis Factor type II, TNFRSF1B, TNFR75, p75, CD120b, TNFR80, p80, sTNF-αcεRII
Amino Acid Sequence MAPEPGSTCR LREYYDQTAQ MCCSKCSPGQ HAKVFCTKTS DTVCDSCEDS TYTQLWNWVP ECLSCGSRCS SDQVETQACT REQNRICTCR PGWYCALSKQ EGCRLCAPLR KCRPGFGVAR PGTETSDVVC KPCAPGTFSN TTSSTDICRP HQICNVVAIP GNASMDAVCT STSP
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Determined by its inhibitory effect of the TNF-alpha mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25 ng/ml of recombinant human TNF-alpha is 0.125 µg/ml.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant human sTNF-α Receptor Type II is an 18.9 kDa protein (174 amino acid residues) comprising the cysteine-rich, ligand binding portion of the extracellular domain of the TNF-αcεRII protein.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source E. coli
Reactivity Human
Research Areas Inflammation; Neurobiology; Receptors; TNF-α Superfamily
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.