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Recombinant Human sTNF Receptor Type I (Carrier-free)
  • Recombinant Human sTNF Receptor Type I (Carrier-free)

Recombinant Human sTNF Receptor Type I (Carrier-free)

Cat No. Size Price Quantity
21-7134-U005 5 µg $76.00
21-7134-U020 20 µg $190.00
21-7134-U100 100 µg $555.00
21-7134-U500 500 µg $1,525.00

Description

TNF-α receptor I (TNFRI), also known as TNFRSF1A and CD120a, is a pro-inflammatory cytokine and member of the TNF-α Receptor superfamily. TNFRI is ubiquitously expressed and stored in the Golgi until receiving inflammatory stimuli. It serves as the main receptor for TNF-alpha, but also binds TNF-αbeta (LT-alpha). TNFRI contains a cytoplasmic death domain that, along with TRADD, participates in activating the classical NF-kB pathway and in initiating apoptosis. Binding of TNF-alpha or –beta to TNFRI results in the activation of this signal transduction pathway. Cleavage or shedding of TNFRI from the cell surface results in a soluble format that is released into general circulation where it binds TNF-α with high affinity, acting as a TNF-α antagonist. sTNFRI has also been associated with various disease states including rheumatoid arthritis.
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TNF-α receptor I (TNFRI), also known as TNFRSF1A and CD120a, is a pro-inflammatory cytokine and member of the TNF-α Receptor superfamily. TNFRI is ubiquitously expressed and stored in the Golgi until receiving inflammatory stimuli. It serves as the main receptor for TNF-alpha, but also binds TNF-αbeta (LT-alpha). TNFRI contains a cytoplasmic death domain that, along with TRADD, participates in activating the classical NF-kB pathway and in initiating apoptosis. Binding of TNF-alpha or –beta to TNFRI results in the activation of this signal transduction pathway. Cleavage or shedding of TNFRI from the cell surface results in a soluble format that is released into general circulation where it binds TNF-α with high affinity, acting as a TNF-α antagonist. sTNFRI has also been associated with various disease states including rheumatoid arthritis.
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Product Details

Name Recombinant Human sTNF-α Receptor Type I (Carrier-free)
Cat. No. 21-7134
Alternative Names TNFRI, TNFRSF1A, CD120a, TNFR55, TNFR60, p55, p55-R, p60
Amino Acid Sequence MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESGSF TASENHLRHC LSCSKCRKEM GQVEISSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity The ED50 is determined by measuring the inhibition of TNF-alpha mediated cytotoxicity in murine L-929 cells. In the presence of 0.25 ng/ml of recombinant human TNF-alpha, the expected ED50 is 0.05 µg/ml.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human sTNF-α receptor I comprises the ligand-binding extracellular domain and is 162 amino acids long with a molecular weight of 18.3 kDa.
Protein Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source E. coli
Reactivity Human
Research Areas TNF-α Superfamily, Apoptosis, Neurobiology, Inflammation, Receptors
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.