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Recombinant Human SPARC (Carrier-Free)
  • Recombinant Human SPARC (Carrier-Free)

Recombinant Human SPARC (Carrier-Free)

Cat No. Size Price Quantity
21-9084-U010 10 µg $80.00
21-9084-U050 50 µg $195.00
21-9084-U100 100 µg $300.00
21-9084-U500 500 µg $900.00

Description

SPARC/Osteonectin is a secreted, evolutionarily-conserved, collagen-binding glycoprotein that is involved in a variety of cellular activities. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair.
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SPARC/Osteonectin is a secreted, evolutionarily-conserved, collagen-binding glycoprotein that is involved in a variety of cellular activities. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair.
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Product Details

Name Recombinant Human SPARC (Carrier-Free)
Cat. No. 21-9084
Alternative Names Secreted protein acidic and rich in cysteine, BM-40, ON
Amino Acid Sequence APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SH
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Determined by its ability to increase alkaline phosphatase activity in differentiating MC3T3 cells using a concentration of 0.5-0.7 ug/ml.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human SPARC/Osteonectin is a glycoprotein containing 286 amino acids that migrates at an apparent MW of 43.7 kDa by SDS-PAGE analysis due to the effect of glycosylation. The calculated molecular weight of Recombinant Human SPARC/Osteonectin is
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source CHO cells
Reactivity Human
Research Areas Proliferation, Wound Healing, Angiogenesis/Cardiovascular, Bones, Skeletal, Cartilage, Cancer
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.