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Recombinant Human Slit2-N (Carrier-free)
  • Recombinant Human Slit2-N (Carrier-free)

Recombinant Human Slit2-N (Carrier-free)

Cat No. Size Price Quantity
21-7132-U005 5 µg $76.00
21-7132-U025 25 µg $175.00
21-7132-U100 100 µg $725.00
21-7132-U500 500 µg $2,295.00

Description

Slit2 belongs to the Slit family of proteins that, along with the Roundabout (Robo) family of receptors, act as guidance cues for cellular migration. In mammals, there are 3 known Slit proteins and 4 Robo receptors. Slit2 associates with Robo1 to contribute to various functions including leukocyte chemotaxis, angiogenesis, neuronal migration and mediating repulsive cues in axon guidance. It has also been reported to inhibit tumor growth and migration in certain types of cancers. Slit2 is initially synthesized as a precursor protein which is subsequently cleaved into N-terminal (Slit2-N) and C-terminal (Slit2-C) fragments. Slit2-N is the larger protein and is associated with neurodevelopment-related activities.
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Slit2 belongs to the Slit family of proteins that, along with the Roundabout (Robo) family of receptors, act as guidance cues for cellular migration. In mammals, there are 3 known Slit proteins and 4 Robo receptors. Slit2 associates with Robo1 to contribute to various functions including leukocyte chemotaxis, angiogenesis, neuronal migration and mediating repulsive cues in axon guidance. It has also been reported to inhibit tumor growth and migration in certain types of cancers. Slit2 is initially synthesized as a precursor protein which is subsequently cleaved into N-terminal (Slit2-N) and C-terminal (Slit2-C) fragments. Slit2-N is the larger protein and is associated with neurodevelopment-related activities.
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Product Details

Name Recombinant Human Slit2-N (Carrier-free)
Cat. No. 21-7132
Alternative Names Slit homolog 2 protein, SLIL3
Amino Acid Sequence QACPAQCSCS GSTVDCHGLA LRSVPRNIPR NTERLDLNGN NITRITKTDF AGLRHLRVLQ LMENKISTIE RGAFQDLKEL ERLRLNRNHL QLFPELLFLG TAKLYRLDLS ENQIQAIPRK AFRGAVDIKN LQLDYNQISC IEDGAFRALR DLEVLTLNNN NITRLSVASF NHMPKLRTFR LHSNNLYCDC HLAWLSDWLR QRPRVGLYTQ CMGPSHLRGH NVAEVQKREF VC
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity The ability to inhibit MC3T3/E1 osteoblast differentiation is measured to determine activity.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human Slit2-N corresponds to the N-terminal portion of full length Slit2. It is 1093 amino acids and has a calculated molecular weight of 122.35 kDa. Due to glycosylation, it migrates at about 120-140 kDa under reducing conditions.
Protein Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source HEK293 cells
Reactivity Human
Research Areas Angiogenesis & Cardiovascular, Chemotaxis, Cancer, Wound Healing, AIDS/HIV, Neurobiology
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.