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Recombinant Human sIL-4Rα (Carrier-free)
  • Recombinant Human sIL-4Rα (Carrier-free)

Recombinant Human sIL-4Rα (Carrier-free)

Cat No. Size Price Quantity
21-7148-U003 3 µg $76.00
21-7148-U015 15 µg $175.00
21-7148-U100 100 µg $775.00
21-7148-U500 500 µg $2,325.00

Description

IL-4R alpha is a Type I transmembrane protein belonging to the class I cytokine receptor family and is a component of two different receptor complexes. When associated with the common gamma chain, which is required for signaling, it binds IL-4 with higher affinity. When complexed with IL-13R alpha I, interaction with both IL-4 and IL-13 is enabled. Through JAK/STAT signal transduction pathways, cell-specific responses including Th2 differentiation, mucosal immunity, allergic inflammation and IgE production result. The soluble form of IL-4R alpha, generated through proteolysis in the human, retains biologic activity and is able to antagonize IL-4.
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IL-4R alpha is a Type I transmembrane protein belonging to the class I cytokine receptor family and is a component of two different receptor complexes. When associated with the common gamma chain, which is required for signaling, it binds IL-4 with higher affinity. When complexed with IL-13R alpha I, interaction with both IL-4 and IL-13 is enabled. Through JAK/STAT signal transduction pathways, cell-specific responses including Th2 differentiation, mucosal immunity, allergic inflammation and IgE production result. The soluble form of IL-4R alpha, generated through proteolysis in the human, retains biologic activity and is able to antagonize IL-4.
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Product Details

Name Recombinant Human sIL-2Rα (Carrier-free)
Cat. No. 21-7148
Alternative Names soluble IL-4 Receptor alpha, CD124, BSF Receptor
Amino Acid Sequence GNMKVLQEPT CVSDYMSIST CEWKMNGPTN CSTELRLLYQ LVFLLSEAHT CIPENNGGAG CVCHLLMDDV VSADNYTLDL WAGQQLLWKG SFKPSEHVKP RAPGNLTVHT NVSDTLLLTW SNPYPPDNYL YNHLTYAVNI WSENDPADFR IYNVTYLEPS LRIAASTLKS GISYRARVRA WAQCYNTTWS EWSPSTKWHN SYREPFEQH
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity The expected ED50 of ≥ 5.0 ng/ml, corresponding to a specific activity of ≥ 2 x 105 units/mg, is measured by determining the ability to inhibit the IL-4 dependent proliferation of human TF-1 cells while in the presence of 0.5 ng/ml I
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human sIL-4Rα corresponds to the entire extracellular domain of IL-4Rα and consists of 209 amino acids.
Protein Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source HEK293 cells
Reactivity Human
Research Areas Immune System, Apoptosis, Cancer, Allergy, Inflammation, Receptors, Differentiation, Proliferation, Stem Cells, Cell Culture, Transplantation
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.