Recombinant Human sIL-2Ra (sCD25) (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-8702-U005 | 5 µg | $76.00 | ||
| 21-8702-U025 | 25 µg | $169.00 | ||
| 21-8702-U100 | 100 µg | $515.00 | ||
| 21-8702-U500 | 500 µg | $1,595.00 |
Technical Data Sheet
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The high-affinity IL-2 receptor is a trimeric complex made up of IL-2R alpha (CD25), IL-2R beta (CD122) and the common gamma chain (IL-2R gamma, CD132). The alpha chain is cleaved from the cell membrane through proteolytic processing to release soluble IL-2R alpha. IL-2R alpha binds IL-2 with low affinity and is not essential for IL-2 signaling, but when combined with the beta and gamma chains it greatly increases affinity for IL-2. IL-2R alpha is expressed during early T and B lymphocyte development and is also induced during mature T and B cell activation. It has been reported to enhance Th17 responses in vitro.
The high-affinity IL-2 receptor is a trimeric complex made up of IL-2R alpha (CD25), IL-2R beta (CD122) and the common gamma chain (IL-2R gamma, CD132). The alpha chain is cleaved from the cell membrane through proteolytic processing to release soluble IL-2R alpha. IL-2R alpha binds IL-2 with low affinity and is not essential for IL-2 signaling, but when combined with the beta and gamma chains it greatly increases affinity for IL-2. IL-2R alpha is expressed during early T and B lymphocyte development and is also induced during mature T and B cell activation. It has been reported to enhance Th17 responses in vitro.
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Product Details
| Name | Recombinant Human sIL-2Rααα (sCD25) (Carrier-free) |
|---|---|
| Cat. No. | 21-8702 |
| Alternative Names | soluble IL-2 receptor, TAC-antigen (p55), CD25 antigen, soluble T cell growth factor receptor (TCGFR) |
| Amino Acid Sequence | ELCDDDPPEI PHATFKAMAY KEGTMLNCEC KRGFRRIKSG SLYMLCTGNS SHSSWDNQCQ CTSSATRNTT KQVTPQPEEQ KERKTTEMQS PMQPVDQASL PGHCREPPPW ENEATERIYH FVVGQMVYYQ CVQGYRALHR GPAESVCKMT HGKTRWTQPQ LICTGEMETS QFPGEEKPQA SPEGRPESET SCLVTTTDFQ IQTEMAATME TSIFTTEYQ |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | The ability to increase the IL-2 proliferative effect on murine CTLL-2 cells is measured. The expected ED50 for this effect, in the presence of ≥ 1ng/ml recombinant IL-2, is between ≥ ≥ ≥ 0.5 - 1.5 µg/ml. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant Human sIL-2Rαα alpha is a 24.8 kDa protein, however due to glycosylation it is observed at about 31 kDa based on SDS-PAGE and Mass Spectrometry. It consists of ≥ 219 amino acid residues, based only on the extracellular domain of IL-2R alpha. |
| Protein Content | Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 98 |
| Source | (BTI-Tn-5B1-4) Hi-5 Insect cells |
| Reactivity | Human |
| Research Areas | AIDS/HIV, Immune System, Inflammation, Neurobiology, Receptors, Transplantation |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.