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Recombinant Human sDLL-1 (Carrier-free)
  • Recombinant Human sDLL-1 (Carrier-free)

Recombinant Human sDLL-1 (Carrier-free)

Cat No. Size Price Quantity
21-7176-U005 5 µg $76.00
21-7176-U025 25 µg $175.00
21-7176-U100 100 µg $585.00
21-7176-U500 500 µg $1,750.00

Description

Delta-like protein 1 (DLL-1) is a type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Upon proteolytic cleavage of DLL-1, the secreted extracellular domain activates the Notch-1 and Notch-2 receptors of adjacent cells. DLL-1 is widely expressed, primarily in the heart, pancreas and epidermis with lower expression in brain and muscle. DLL-1 mediated notch signaling is required for embryonic development as well the maintenance of adult stem cells. In the immune system DLL-1 promotes development of T-cell/NK-cell precursor cells by blocking the differentiation of progenitor cells into the B-cell lineage. Human sDLL-1 has been shown to suppress apoptosis and promote hematopoietic progenitor cells expansion in cell culture. Human sDLL-1 allows monocyte differentiation into dendritic cells but blocks monocyte differentiation into macrophages.
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Delta-like protein 1 (DLL-1) is a type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Upon proteolytic cleavage of DLL-1, the secreted extracellular domain activates the Notch-1 and Notch-2 receptors of adjacent cells. DLL-1 is widely expressed, primarily in the heart, pancreas and epidermis with lower expression in brain and muscle. DLL-1 mediated notch signaling is required for embryonic development as well the maintenance of adult stem cells. In the immune system DLL-1 promotes development of T-cell/NK-cell precursor cells by blocking the differentiation of progenitor cells into the B-cell lineage. Human sDLL-1 has been shown to suppress apoptosis and promote hematopoietic progenitor cells expansion in cell culture. Human sDLL-1 allows monocyte differentiation into dendritic cells but blocks monocyte differentiation into macrophages.
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Product Details

Name Recombinant Human sDLL-1 (Carrier-free)
Cat. No. 21-7176
Alternative Names soluble DLL-1, Delta-like protein 1, Delta-1
Amino Acid Sequence SGVFELKLQE FVNKKGLLGN RNCCRGGAGP PPCACRTFFR VCLKHYQASV SPEPPCTYGS AVTPVLGVDS FSLPDGGGAD SAFSNPIRFP FGFTWPGTFS LIIEALHTDS PDDLATENPE RLISRLATQR HLTVGEEWSQ DLHSSGRTDL KYSYRFVCDE HYYGEGCSVF CRPRDDAFGH FTCGERGEKV CNPGWKGPYC TEPICLPGCD EQHGFCDKPG ECKCRVGWQG RY
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Determined by the dose dependent growth suppression of the human acute monocytic leukemia cell line, THP-1. sDLL-1 inhibits the proliferation in THP-1 cells using a concentration of 3.0-5.0 μg/ml.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human sDLL-1 is the 56.3 kDa soluble extracellular signaling domain of the DLL-1 protein.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 95
Source HEK293 cells
Reactivity Human
Research Areas Angiogenesis & Cardiovascular, Cancer, Proliferation
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.