Recombinant Human SCF (Carrier-Free)
  • Recombinant Human SCF (Carrier-Free)

Recombinant Human SCF (Carrier-Free)

Cat No. Size Price Quantity
21-9143-U002 2 µg $80.00
21-9143-U010 10 µg $195.00
21-9143-U050 50 µg $288.00
21-9143-U100 100 µg $480.00
Lead time: 2-3 business days

Description

SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on Mouse cells, while Mouse and rat SCF are fully active on human cells.
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SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on Mouse cells, while Mouse and rat SCF are fully active on human cells.
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Product Details

Name Recombinant Human SCF (Carrier-Free)
Cat. No. 21-9143
Alternative Names Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor
Amino Acid Sequence MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ? 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.
Endotoxin Level The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ? 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.
Molecular Mass Recombinant Human SCF is an 18.4 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source E. coli
Reactivity Human
Research Areas Immune System, Proliferation, Stem Cells & Differentiation, Cell Culture
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.