Recombinant Human sCD40 Ligand (Carrier-free)
|Name||Recombinant Human sCD40 Ligand (Carrier-free)|
|Alternative Names||soluble CD40 Ligand, TNF-αSF5, TRAP, CD154, gp39, T-BAM|
|Amino Acid Sequence||MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL|
|Authenticity||Verified by N-terminal and Mass Spectrometry analyses (when applicable).|
Assay #1: Determined by the stimulation of IL-12 induction by human PBMCs.
Assay #2: Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The expected ED50 for this effect is 5-
|Endotoxin Level||Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).|
|Molecular Mass||Recombinant human soluble CD40 ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-αlike domain of CD40L.|
|Protein Content||Content Verified by UV Spectroscopy and/or SDS-PAGE gel.|
|Research Areas||Angiogenesis/Cardiovascular; Apoptosis; Immune System; Inflammation; Proliferation; TNF-α Superfamily; Allergy; Transplantation|
|Reconstitution||See Certificate of Analysis (COA) for lot specific reconstitution information.|
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.