Recombinant Human sCD40 Ligand (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-7088-U010 | 10 µg | $69.00 | ||
| 21-7088-U050 | 50 µg | $295.00 | ||
| 21-7088-U500 | 500 µg | $829.00 |
Technical Data Sheet
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CD40 Ligand (CD40L, CD154, TRAP, TNF-αSF5, gp39) belongs to the TNF-α superfamily and in its soluble form, displays some cytokine-like properties. CD40L binds to its receptor, CD40, to mediate T cell-dependent B cell responses and contribute to monocyte activation and dendritic cell maturation. Membrane-bound CD40L is expressed mainly on activated T cells, a small population of CD8+ T cells, and has been demonstrated on mast cells, basophils, eosinophils, B cells, NK cells and monocytes/macrophages. Activated platelets express CD40L on their surface and constitute the major source of soluble CD40L (sCD40L). sCD40L is a shorter protein than the membrane-bound version and retains biologic activity and ability to form trimers. CD40L expression can be upregulated using mitogens like PMA or PHA, through anti-CD3 activation, or by thrombin in platelets.
CD40 Ligand (CD40L, CD154, TRAP, TNF-αSF5, gp39) belongs to the TNF-α superfamily and in its soluble form, displays some cytokine-like properties. CD40L binds to its receptor, CD40, to mediate T cell-dependent B cell responses and contribute to monocyte activation and dendritic cell maturation. Membrane-bound CD40L is expressed mainly on activated T cells, a small population of CD8+ T cells, and has been demonstrated on mast cells, basophils, eosinophils, B cells, NK cells and monocytes/macrophages. Activated platelets express CD40L on their surface and constitute the major source of soluble CD40L (sCD40L). sCD40L is a shorter protein than the membrane-bound version and retains biologic activity and ability to form trimers. CD40L expression can be upregulated using mitogens like PMA or PHA, through anti-CD3 activation, or by thrombin in platelets.
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Product Details
| Name | Recombinant Human sCD40 Ligand (Carrier-free) |
|---|---|
| Cat. No. | 21-7088 |
| Alternative Names | soluble CD40 Ligand, TNF-αSF5, TRAP, CD154, gp39, T-BAM |
| Amino Acid Sequence | MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity |
Assay #1: Determined by the stimulation of IL-12 induction by human PBMCs. Assay #2: Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The expected ED50 for this effect is 5- |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant human soluble CD40 ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-αlike domain of CD40L. |
| Protein Content | Content Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 98 |
| Source | E. coli |
| Reactivity | Human |
| Research Areas | Angiogenesis/Cardiovascular; Apoptosis; Immune System; Inflammation; Proliferation; TNF-α Superfamily; Allergy; Transplantation |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.