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Recombinant Human sCD30 Ligand (Carrier-free)
  • Recombinant Human sCD30 Ligand (Carrier-free)

Recombinant Human sCD30 Ligand (Carrier-free)

Cat No. Size Price Quantity
21-7143-U010 10 µg $69.00
21-7143-U050 50 µg $169.00
21-7143-U100 100 µg $280.00
21-7143-U500 500 µg $859.00

Description

CD30 ligand, also known as CD153 and TNF-αSF8, is a Type II glycoprotein and a member of the TNF-α superfamily. It is expressed on the surface of activated B cell subsets, T cells and monocytes. In the presence of CD30, CD30L is cleaved and the soluble form binds to CD30+ cells which can induce apoptosis in some cases. CD30L/CD30 interactions also generate signaling pathways leading to either cell death or proliferation. Studies suggest a role for CD30L/CD30 signaling in Th1, Th2 and Th17 responses, as well as in certain diseases such as allergic inflammation, rheumatoid arthritis and Hodgkin's lymphoma.
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CD30 ligand, also known as CD153 and TNF-αSF8, is a Type II glycoprotein and a member of the TNF-α superfamily. It is expressed on the surface of activated B cell subsets, T cells and monocytes. In the presence of CD30, CD30L is cleaved and the soluble form binds to CD30+ cells which can induce apoptosis in some cases. CD30L/CD30 interactions also generate signaling pathways leading to either cell death or proliferation. Studies suggest a role for CD30L/CD30 signaling in Th1, Th2 and Th17 responses, as well as in certain diseases such as allergic inflammation, rheumatoid arthritis and Hodgkin's lymphoma.
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Product Details

Name Recombinant Human sCD30 Ligand (Carrier-free)
Cat. No. 21-7143
Alternative Names CD30L, TNF-αSF8, CD153
Amino Acid Sequence HHHHHHHHPS PGGSGGQRTD SIPNSPDNVP LKGGNCSEDL LCILKRAPFK KSWAYLQVAK HLNKTKLSWN KDGILHGVRY QDGNLVIQFP GLYFIICQLQ FLVQCPNNSV DLKLELLINK HIKKQALVTV CESGMQTKHV YQNLSQFLLD YLQVNTTISV NVDTFQYIDT STFPLENVLS IFLYSNSD
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Using a concentration range of 10.0-25.0 ng/ml, the ability to stimulate human IL-8 production in human PBMC is determined. Results may vary due to differences between ≥ ≥ ≥ PBMC donors.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human soluble CD30L consists of 188 amino acids, corresponding to the extracellular domain plus an 8 residue N-terminal His-Tag. It has an approximate molecular weight of ≥ 21.3 kDa.
Protein Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source CHO cells
Reactivity Human
Research Areas Cancer, TNF-α Superfamily, Immune System, Allergy, Inflammation, Diabetes
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.