Recombinant Human sCD30 Ligand (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-7143-U010 | 10 µg | $69.00 | ||
| 21-7143-U050 | 50 µg | $169.00 | ||
| 21-7143-U100 | 100 µg | $280.00 | ||
| 21-7143-U500 | 500 µg | $859.00 |
Technical Data Sheet
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CD30 ligand, also known as CD153 and TNF-αSF8, is a Type II glycoprotein and a member of the TNF-α superfamily. It is expressed on the surface of activated B cell subsets, T cells and monocytes. In the presence of CD30, CD30L is cleaved and the soluble form binds to CD30+ cells which can induce apoptosis in some cases. CD30L/CD30 interactions also generate signaling pathways leading to either cell death or proliferation. Studies suggest a role for CD30L/CD30 signaling in Th1, Th2 and Th17 responses, as well as in certain diseases such as allergic inflammation, rheumatoid arthritis and Hodgkin's lymphoma.
CD30 ligand, also known as CD153 and TNF-αSF8, is a Type II glycoprotein and a member of the TNF-α superfamily. It is expressed on the surface of activated B cell subsets, T cells and monocytes. In the presence of CD30, CD30L is cleaved and the soluble form binds to CD30+ cells which can induce apoptosis in some cases. CD30L/CD30 interactions also generate signaling pathways leading to either cell death or proliferation. Studies suggest a role for CD30L/CD30 signaling in Th1, Th2 and Th17 responses, as well as in certain diseases such as allergic inflammation, rheumatoid arthritis and Hodgkin's lymphoma.
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Product Details
| Name | Recombinant Human sCD30 Ligand (Carrier-free) |
|---|---|
| Cat. No. | 21-7143 |
| Alternative Names | CD30L, TNF-αSF8, CD153 |
| Amino Acid Sequence | HHHHHHHHPS PGGSGGQRTD SIPNSPDNVP LKGGNCSEDL LCILKRAPFK KSWAYLQVAK HLNKTKLSWN KDGILHGVRY QDGNLVIQFP GLYFIICQLQ FLVQCPNNSV DLKLELLINK HIKKQALVTV CESGMQTKHV YQNLSQFLLD YLQVNTTISV NVDTFQYIDT STFPLENVLS IFLYSNSD |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | Using a concentration range of 10.0-25.0 ng/ml, the ability to stimulate human IL-8 production in human PBMC is determined. Results may vary due to differences between ≥ ≥ ≥ PBMC donors. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant Human soluble CD30L consists of 188 amino acids, corresponding to the extracellular domain plus an 8 residue N-terminal His-Tag. It has an approximate molecular weight of ≥ 21.3 kDa. |
| Protein Content | Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 98 |
| Source | CHO cells |
| Reactivity | Human |
| Research Areas | Cancer, TNF-α Superfamily, Immune System, Allergy, Inflammation, Diabetes |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.