Recombinant Human sCD27 Ligand (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-7142-U010 | 10 µg | $69.00 | ||
| 21-7142-U050 | 50 µg | $169.00 | ||
| 21-7142-U100 | 100 µg | $280.00 | ||
| 21-7142-U500 | 500 µg | $859.00 |
Technical Data Sheet
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CD27 ligand, also known as CD70, is a type II transmembrane protein belonging to the TNF-α superfamily. It is predominantly expressed on NK cells and upon activation is induced on T and B lymphocytes and certain dendritic cell subsets. CD27L is an important co-stimulatory molecule involved in proliferation and clonal expansion of T cells, and provides an alternative signal for T cell activation. Interactions between ≥ ≥ ≥ CD27 and its ligand particularly enhance the CD8+ T cell response, but may also play a role in CD4+ T cell responses.
CD27 ligand, also known as CD70, is a type II transmembrane protein belonging to the TNF-α superfamily. It is predominantly expressed on NK cells and upon activation is induced on T and B lymphocytes and certain dendritic cell subsets. CD27L is an important co-stimulatory molecule involved in proliferation and clonal expansion of T cells, and provides an alternative signal for T cell activation. Interactions between ≥ ≥ ≥ CD27 and its ligand particularly enhance the CD8+ T cell response, but may also play a role in CD4+ T cell responses.
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Product Details
| Name | Recombinant Human sCD27 Ligand (Carrier-free) |
|---|---|
| Cat. No. | 21-7142 |
| Alternative Names | CD27L, TNF-αSF7, CD70, Ki-24 antigen |
| Amino Acid Sequence | HHHHHHHHPS PGGSGGQRFA QAQQQLPLES LGWDVAELQL NHTGPQQDPR LYWQGGPALG RSFLHGPELD KGQLRIHRDG IYMVHIQVTL AICSSTTASR HHPTTLAVGI CSPASRSISL LRLSFHQGCT IASQRLTPLA RGDTLCTNLT GTLLPSRNTD ETFFGVQWVR P |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | Using a concentration range of 10.0-25.0 ng/ml, the ability to stimulate human IL-8 production in human PBMC is determined. Results may vary due to differences between ≥ ≥ ≥ PBMC donors. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant Human sCD27L has a calculated molecular weight of 18.8 kDa and consists of the 155 amino acid extracellular domain plus an N-terminal His-Tag. |
| Protein Content | Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 95 |
| Source | CHO cells |
| Reactivity | Human |
| Research Areas | TNF-α Superfamily, Immune System |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.