Recombinant Human sCD23 (FceRII) (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-7141-U005 | 5 µg | $76.00 | ||
| 21-7141-U020 | 20 µg | $175.00 | ||
| 21-7141-U100 | 100 µg | $585.00 | ||
| 21-7141-U500 | 500 µg | $1,750.00 |
Lead time: 2-3 business days
Technical Data Sheet
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CD23, also known as Fc epsilon RII (FceRII), is a low affinity receptor for IgE and belongs to the C-type lectin superfamily. It is expressed on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets. Soluble CD23 is generated through cysteine-protease or metalloprotease activity. A long (a) form is expressed consitutively on B cells, while a short (b) form is upregulated by IL-4 during allergic inflammation. Fragments of different sizes are generated and function may be related to fragment size, as well as form. CD23 plays a role IgE regulation, isotype switching, B cell homeostasis and stimulating monocytes to release pro-inflammatory cytokines.
CD23, also known as Fc epsilon RII (FceRII), is a low affinity receptor for IgE and belongs to the C-type lectin superfamily. It is expressed on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets. Soluble CD23 is generated through cysteine-protease or metalloprotease activity. A long (a) form is expressed consitutively on B cells, while a short (b) form is upregulated by IL-4 during allergic inflammation. Fragments of different sizes are generated and function may be related to fragment size, as well as form. CD23 plays a role IgE regulation, isotype switching, B cell homeostasis and stimulating monocytes to release pro-inflammatory cytokines.
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Product Details
| Name | Recombinant Human sCD23 (FceRII) (Carrier-free) |
|---|---|
| Cat. No. | 21-7141 |
| Alternative Names | soluble CD23, Fc epsilon RII, Lymphocyte IgE receptor, BLAST-2, CLEC4J, IGEBF, Ly-42 |
| Amino Acid Sequence | MELQVSSGFV CNTCPEKWIN FQRKCYYFGK GTKQWVHARY ACDDMEGQLV SIHSPEEQDF LTKHASHTGS WIGLRNLDLK GEFIWVDGSH VDYSNWAPGE PTSRSQGEDC VMMRGSGRWN DAFCDRKLGA WVCDRLATCT PPASEGSAES MGPDSRPDPD GRLPTPSAPL HS |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | The ability to induce TNF-alpha production in human PBMCs is measured. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant Human sCD23 consists of 172 amino-acids. This results in a 19.2 kDa non-glycosylated protein. |
| Protein Content | Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 96 |
| Source | E. coli |
| Reactivity | Human |
| Research Areas | Inflammation, Immune System, Receptors |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.