Recombinant Human sCD23 (FceRII) (Carrier-free)
  • Recombinant Human sCD23 (FceRII) (Carrier-free)

Recombinant Human sCD23 (FceRII) (Carrier-free)

Cat No. Size Price Quantity
21-7141-U005 5 µg $76.00
21-7141-U020 20 µg $175.00
21-7141-U100 100 µg $585.00
21-7141-U500 500 µg $1,750.00
Lead time: 2-3 business days

Description

CD23, also known as Fc epsilon RII (FceRII), is a low affinity receptor for IgE and belongs to the C-type lectin superfamily. It is expressed on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets. Soluble CD23 is generated through cysteine-protease or metalloprotease activity. A long (a) form is expressed consitutively on B cells, while a short (b) form is upregulated by IL-4 during allergic inflammation. Fragments of different sizes are generated and function may be related to fragment size, as well as form. CD23 plays a role IgE regulation, isotype switching, B cell homeostasis and stimulating monocytes to release pro-inflammatory cytokines.
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CD23, also known as Fc epsilon RII (FceRII), is a low affinity receptor for IgE and belongs to the C-type lectin superfamily. It is expressed on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets. Soluble CD23 is generated through cysteine-protease or metalloprotease activity. A long (a) form is expressed consitutively on B cells, while a short (b) form is upregulated by IL-4 during allergic inflammation. Fragments of different sizes are generated and function may be related to fragment size, as well as form. CD23 plays a role IgE regulation, isotype switching, B cell homeostasis and stimulating monocytes to release pro-inflammatory cytokines.
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Product Details

Name Recombinant Human sCD23 (FceRII) (Carrier-free)
Cat. No. 21-7141
Alternative Names soluble CD23, Fc epsilon RII, Lymphocyte IgE receptor, BLAST-2, CLEC4J, IGEBF, Ly-42
Amino Acid Sequence MELQVSSGFV CNTCPEKWIN FQRKCYYFGK GTKQWVHARY ACDDMEGQLV SIHSPEEQDF LTKHASHTGS WIGLRNLDLK GEFIWVDGSH VDYSNWAPGE PTSRSQGEDC VMMRGSGRWN DAFCDRKLGA WVCDRLATCT PPASEGSAES MGPDSRPDPD GRLPTPSAPL HS
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity The ability to induce TNF-alpha production in human PBMCs is measured.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human sCD23 consists of 172 amino-acids. This results in a 19.2 kDa non-glycosylated protein.
Protein Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 96
Source E. coli
Reactivity Human
Research Areas Inflammation, Immune System, Receptors
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.