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Recombinant Human sCD22 (Siglec-2) (Carrier-free)
  • Recombinant Human sCD22 (Siglec-2) (Carrier-free)

Recombinant Human sCD22 (Siglec-2) (Carrier-free)

Cat No. Size Price Quantity
21-7140-U005 5 µg $76.00
21-7140-U020 20 µg $179.00
21-7140-U100 100 µg $800.00
21-7140-U500 500 µg $2,750.00
Lead time: 2-3 business days

Description

sCD22, also known as Siglec-2 and BL-CAM, is a cell surface glycoprotein that is B-lineage restricted. It is expressed on the surface of mature B-cells and, to some extent, on immature B-cells. Siglec lectins belong to the immunoglobulin (Ig) superfamily. sCD22 is a coreceptor that binds the B-cell receptor (BCR) and acts as a negative regulator in B-cell receptor signal transduction. Loss of CD22 function may contribute to the pathogenesis of autoimmune diseases.
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sCD22, also known as Siglec-2 and BL-CAM, is a cell surface glycoprotein that is B-lineage restricted. It is expressed on the surface of mature B-cells and, to some extent, on immature B-cells. Siglec lectins belong to the immunoglobulin (Ig) superfamily. sCD22 is a coreceptor that binds the B-cell receptor (BCR) and acts as a negative regulator in B-cell receptor signal transduction. Loss of CD22 function may contribute to the pathogenesis of autoimmune diseases.
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Product Details

Name Recombinant Human sCD22 (Siglec-2) (Carrier-free)
Cat. No. 21-7140
Alternative Names soluble CD22, Leu-14, B-lymphocyte cell adhesion molecule, BL-CAM, Siglec-2
Amino Acid Sequence SKWVFEHPET LYAWEGACVW IPCTYRALDG DLESFILFHN PEYNKNTSKF DGTRLYESTK DGKVPSEQKR VQFLGDKNKN CTLSIHPVHL NDSGQLGLRM ESKTEKWMER IHLNVSERPF PPHIQLPPEI QESQEVTLTC LLNFSCYGYP IQLQWLLEGV PMRQAAVTST SLTIKSVFTR SELKFSPQWS HHGKIVTCQL QDADGKFLSN DTVQLNVKHT PKLEIKVTPS DA
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity The expected ED50 of 10-17 μg/ml is determined by its capability to inhibit proliferation of Raji cells.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human CD22 corresponds to the 660+ amino acid extracellular domain, which results in a soluble 75.0 kDa protein.
Protein Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source CHO cells
Reactivity Human
Research Areas Immune System, Cancer
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.