Recombinant Human sCD14 (Carrier-free)
  • Recombinant Human sCD14 (Carrier-free)

Recombinant Human sCD14 (Carrier-free)

Cat No. Size Price Quantity
21-7173-U010 10 µg $69.00
21-7173-U050 50 µg $175.00
21-7173-U100 100 µg $280.00
21-7173-U500 500 µg $859.00
Lead time: 2-3 business days

Description

CD14, expressed mainly by monocytes and macrophages, is generally present as a membrane-bound surface glycoprotein. It serves as a homing receptor for lipopolysaccharide (LPS) and is a pattern recognition receptor for a variety of microbial-derived ligands. CD14 is not a signaling protein and forms a receptor complex with toll-like receptor 4 (TLR4) to transduce CD14-LPS signals. CD14 is also present as a soluble format in serum and other bodily fluids (sCD14). This soluble format can be shed from the cell surface and may play a role in inhibiting LPS-mediated responses in extracellular spaces.
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CD14, expressed mainly by monocytes and macrophages, is generally present as a membrane-bound surface glycoprotein. It serves as a homing receptor for lipopolysaccharide (LPS) and is a pattern recognition receptor for a variety of microbial-derived ligands. CD14 is not a signaling protein and forms a receptor complex with toll-like receptor 4 (TLR4) to transduce CD14-LPS signals. CD14 is also present as a soluble format in serum and other bodily fluids (sCD14). This soluble format can be shed from the cell surface and may play a role in inhibiting LPS-mediated responses in extracellular spaces.
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Product Details

Name Recombinant Human sCD14 (Carrier-free)
Cat. No. 21-7173
Alternative Names soluble CD14, Monocyte Differentiation Antigen CD14
Amino Acid Sequence TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QP
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Using a concentration range of ≥ 20 - 200 ng/ul, dose dependent activation of NF-kB in a RAW264 cell line based reporter system is measured. In the presence of 0.25 - 1.0 ng/ul of bacterial LPS enhances the activation assay.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human sCD14 is a 35.6 kDa protein comprising 331 amino acids of the extracellular portion of the CD14 receptor.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 95
Source HEK293 cells
Reactivity Human
Research Areas Receptors, Inflammation
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.