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Recombinant Human sCD100 (Sema4D) (Carrier-free)
  • Recombinant Human sCD100 (Sema4D) (Carrier-free)

Recombinant Human sCD100 (Sema4D) (Carrier-free)

Cat No. Size Price Quantity
21-7144-U005 5 µg $76.00
21-7144-U020 20 µg $179.00
21-7144-U100 100 µg $800.00
21-7144-U500 500 µg $2,995.00

Description

CD100, also known as Sema4D, is a transmembrane protein belonging to the Class IV subfamily of Semaphorins. CD100 is active as a homodimer, however cleavage by certain metalloproteinases produce a soluble dimer that is also active. It is expressed on T and B cells, as well as macrophages, and with upregulated expression on activated T cells. CD100 plays important roles in immune and inflammatory responses, being involved in B cell and dendritic cell activation. It also induces B cells to aggregate and may improve their viability. CD72 is one of ≥ 2 known receptors for CD100 and binding of the ligand to this receptor is reported to be an important factor in maintaining immunological homeostasis.
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CD100, also known as Sema4D, is a transmembrane protein belonging to the Class IV subfamily of Semaphorins. CD100 is active as a homodimer, however cleavage by certain metalloproteinases produce a soluble dimer that is also active. It is expressed on T and B cells, as well as macrophages, and with upregulated expression on activated T cells. CD100 plays important roles in immune and inflammatory responses, being involved in B cell and dendritic cell activation. It also induces B cells to aggregate and may improve their viability. CD72 is one of ≥ 2 known receptors for CD100 and binding of the ligand to this receptor is reported to be an important factor in maintaining immunological homeostasis.
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Product Details

Name Recombinant Human sCD100 (Sema4D) (Carrier-free)
Cat. No. 21-7144
Alternative Names Semaphorin 4D, SEMAJ, M-sema-G
Amino Acid Sequence FAPIPRITWE HREVHLVQFH EPDIYNYSAL LLSEDKDTLY IGAREAVFAV NALNISEKQH EVYWKVSEDK KAKCAEKGKS KQTECLNYIR VLQPLSATSL YVCGTNAFQP ACDHLNLTSF KFLGKNEDGK GRCPFDPAHS YTSVMVDGEL YSGTSYNFLG SEPIISRNSS HSPLRTEYAI PWLNEPSFVF ADVIRKSPDS PDGEDDRVYF FFTEVSVEYE FVFRVLIPRI AR
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Inhibition of chemokine-induced (hMCP-3) mobility of human monocytes is measured.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human sCD100 is 711 amino acids, comprising the protein's extracellular domain. It has a molecular weight of 78.9 kDa and appears as a mix of disulfide-linked dimer and monomer when SDS-PAGE analysis is performed under non-reducing conditions.
Protein Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 97
Source CHO cells
Reactivity Human
Research Areas Immune System, Chemotaxis
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.