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Recombinant Human sAPO-1/Fas (Carrier-free)
  • Recombinant Human sAPO-1/Fas (Carrier-free)

Recombinant Human sAPO-1/Fas (Carrier-free)

Cat No. Size Price Quantity
21-7083-U005 5 µg $69.00
21-7083-U020 20 µg $175.00
21-7083-U100 100 µg $675.00

Description

Fas (APO-1, CD95, TNFRSF6) is a member of the TNF-α receptor superfamily and through alternative splicing, exists in multiple isoforms including a soluble form. Binding of Fas to its receptor, Fas ligand (FasL), triggers apoptosis through the recruitment of FADD and activation of the caspase cascade. sFas is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. Fas expression has been detected in a variety of cell types including activated T and B cells, fibroblasts, dendritic cells, thymocytes, macrophages, hepatocytes, cardiomyocytes and malignant human lymphoid cell lines.
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Fas (APO-1, CD95, TNFRSF6) is a member of the TNF-α receptor superfamily and through alternative splicing, exists in multiple isoforms including a soluble form. Binding of Fas to its receptor, Fas ligand (FasL), triggers apoptosis through the recruitment of FADD and activation of the caspase cascade. sFas is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. Fas expression has been detected in a variety of cell types including activated T and B cells, fibroblasts, dendritic cells, thymocytes, macrophages, hepatocytes, cardiomyocytes and malignant human lymphoid cell lines.
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Product Details

Name Recombinant Human sAPO-1/Fas (Carrier-free)
Cat. No. 21-7083
Alternative Names soluble Fas receptor (sFasR), TNFRSF6, CD95, Apo I, Fas Antigen
Amino Acid Sequence MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity The ED50 was determined by its ability to inhibit the cytotoxicity of Jurkat cells is between ≥ ≥ ≥ 10-15 µg/ml in the presence of ≥ 2 ng/ml of hFasL.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant human soluble Fas is a 157 amino acid polypeptide (17.6 kDa) corresponding to the TNFR-homologous cysteine-rich extracellular Fas domain.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source E. coli
Reactivity Human
Research Areas AIDS/HIV; Receptors; TNF-α Superfamily
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.