Recombinant Human R-Spondin 2 (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-7127-U005 | 5 µg | $76.00 | ||
| 21-7127-U020 | 20 µg | $175.00 | ||
| 21-7127-U100 | 100 µg | $585.00 | ||
| 21-7127-U500 | 500 µg | $1,750.00 |
Technical Data Sheet
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R-Spondin 2 (RSPO2) is a secreted protein belonging to the R-Spondin family of four structurally related Wnt/beta-catenin signaling regulators. All four members contain one thrombospondin-like and two furin-like domains. In adults, R-Spondin 2 is expressed in intestine and lung as well as other organs of endodermal origin. R-Spondins can enhance Wnt/beta-catenin signaling as they compete with the Wnt agonist DKK-1 for binding to Wnt co-receptors LRP6/Kremen. They can also act as a signaling activation ligand on LRP6 and LGR4 to form a cluster with WNT and FZD. The R-Spondins have been demonstrated to bind to LGR-4, LGR-5 and LGR-6 receptors with high affinity.
R-Spondin 2 (RSPO2) is a secreted protein belonging to the R-Spondin family of four structurally related Wnt/beta-catenin signaling regulators. All four members contain one thrombospondin-like and two furin-like domains. In adults, R-Spondin 2 is expressed in intestine and lung as well as other organs of endodermal origin. R-Spondins can enhance Wnt/beta-catenin signaling as they compete with the Wnt agonist DKK-1 for binding to Wnt co-receptors LRP6/Kremen. They can also act as a signaling activation ligand on LRP6 and LGR4 to form a cluster with WNT and FZD. The R-Spondins have been demonstrated to bind to LGR-4, LGR-5 and LGR-6 receptors with high affinity.
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Product Details
| Name | Recombinant Human R-Spondin 2 (Carrier-free) |
|---|---|
| Cat. No. | 21-7127 |
| Alternative Names | Roof plate-specific spondin-2, RSPO2, Cristin 2 |
| Amino Acid Sequence | ASYVSNPICK GCLSCSKDNG CSRCQQKLFF FLRREGMRQY GECLHSCPSG YYGHRAPDMN RCARCRIENC DSCFSKDFCT KCKVGFYLHR GRCFDECPDG FAPLEETMEC VEGCEVGHWS EWGTCSRNNR TCGFKWGLET RTRQIVKKPV KDTILCPTIA ESRRCKMTMR HCPGGKRTPK AKEKRNKKKK RKLIERAQEQ HSVFLATDRA NQ |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | The expected ED50 is 0.8-2.0 ug/ml, based on measuring the R-Spondin-2 enhancement of BMP-2-mediated differentiation of MC3T3-E1 cells. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant Human R-Spondin 2 is a 212 amino acid protein of ≥ 24.4 kDa, however due to glycosylation it migrates at about 30 kDa under reducing conditions. |
| Protein Content | Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 95 |
| Source | CHO cells |
| Reactivity | Human |
| Research Areas | Cancer, Proliferation, Neurobiology |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.