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Recombinant Human R-Spondin 1 (Carrier-free)
  • Recombinant Human R-Spondin 1 (Carrier-free)

Recombinant Human R-Spondin 1 (Carrier-free)

Cat No. Size Price Quantity
21-7126-U005 5 µg $76.00
21-7126-U020 20 µg $175.00
21-7126-U100 100 µg $585.00
21-7126-U500 500 µg $1,750.00

Description

R-Spondin 1 (RSPO1) is a secreted protein belonging to the R-Spondin family of four structurally related Wnt/beta-catenin signaling regulators. All four members contain one thrombospondin-like and two furin-like domains. During early development, R-Spondin 1 is thought to play a role in neural tube development. It has also been shown to be involved in female gonad determination. R-Spondins can enhance Wnt/beta-catenin signaling as they compete with the Wnt agonist DKK-1 for binding to Wnt co-receptors LRP6/Kremen. They can also act as a signaling activation ligand on LRP6 and LGR4 to form a cluster with WNT and FZD. The R-Spondins have been demonstrated to bind to LGR-4, LGR-5 and LGR-6 receptors with high affinity.
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R-Spondin 1 (RSPO1) is a secreted protein belonging to the R-Spondin family of four structurally related Wnt/beta-catenin signaling regulators. All four members contain one thrombospondin-like and two furin-like domains. During early development, R-Spondin 1 is thought to play a role in neural tube development. It has also been shown to be involved in female gonad determination. R-Spondins can enhance Wnt/beta-catenin signaling as they compete with the Wnt agonist DKK-1 for binding to Wnt co-receptors LRP6/Kremen. They can also act as a signaling activation ligand on LRP6 and LGR4 to form a cluster with WNT and FZD. The R-Spondins have been demonstrated to bind to LGR-4, LGR-5 and LGR-6 receptors with high affinity.
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Product Details

Name Recombinant Human R-Spondin 1 (Carrier-free)
Cat. No. 21-7126
Alternative Names Roof plate-specific spondin-1, RSPO1, Cristin 3
Amino Acid Sequence SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QG
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity The expected ED50 is 1.0-3.0 ug/ml, based on measuring the R-Spondin-1 enhancement of BMP-2-mediated differentiation of MC3T3-E1 cells.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human R-Spondin 1 is a 243 amino acid protein with a molecular weight of ≥ 26.7 kDa. Due to glycosylation, it migrates at about 40 kDa under reducing conditions.
Protein Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 95
Source CHO cells
Reactivity Human
Research Areas Cancer, Proliferation, Neurobiology
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.