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Recombinant Human PECAM-I (Carrier-Free)
  • Recombinant Human PECAM-I (Carrier-Free)

Recombinant Human PECAM-I (Carrier-Free)

Cat No. Size Price Quantity
21-9214-U010 10 µg $80.00
21-9214-U050 50 µg $195.00
21-9214-U100 100 µg $300.00
21-9214-U500 500 µg $900.00

Description

PECAM is transmembrane glycoprotein that belongs to the Ig-related superfamily of adhesion molecules. It is highly expressed at endothelial cell junctions, and is also expressed in platelets and most leukocyte sub-types. Recombinant Human PECAM-1 is a 572 amino acid glycoprotein comprising the extracellular domain of PECAM-1.
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PECAM is transmembrane glycoprotein that belongs to the Ig-related superfamily of adhesion molecules. It is highly expressed at endothelial cell junctions, and is also expressed in platelets and most leukocyte sub-types. Recombinant Human PECAM-1 is a 572 amino acid glycoprotein comprising the extracellular domain of PECAM-1.
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Product Details

Name Recombinant Human PECAM-I (Carrier-Free)
Cat. No. 21-9214
Alternative Names Platelet endothelial cell adhesion molecule, CD31 antigen, EndoCAM
Amino Acid Sequence ENSFTINSV DMKSLPDWTV QNGKNLTLQC FADVSTTSHV KPQHQMLFYK DDVLFYNISS MKSTESYFIP EVRIYDSGTY KCTVIVNNKE KTTAEYQLLV EGVPSPRVTL DKKEAIQGGI VRVNCSVPEE KAPIHFTIEK LELNEKMVKL KREKNSRDQN FVILEFPVEE QDRVLSFRCQ ARIISGIHMQ TSESTKSELV TVTESFSTPK FHISPTGMIM EGAQLHIKCT IQV
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Determined by its ability to support the adhesion of activated Jurkat cells. The expected ED50 for this effect is 1.0 -1.5 ug/ml.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Monomeric glycosylated PECAM-1 migrates at an apparent molecular weight of approximately 80.0-95.0 kDa by SDS-PAGE analysis under reducing conditions. The calculated molecular weight of Recombinant Human PECAM-1 is 64.3 kDa.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 97
Source HEK293 cells
Reactivity Human
Research Areas Inflammation
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.