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Recombinant Human PDGF-AA (Carrier-free)
  • Recombinant Human PDGF-AA (Carrier-free)

Recombinant Human PDGF-AA (Carrier-free)

Cat No. Size Price Quantity
21-7080-U002 2 µg $69.00
21-7080-U010 10 µg $179.00
21-7080-U100 100 µg $769.00
21-7080-U500 500 µg $2,295.00

Description

Platelet-derived growth factor-AA (PDGF-AA) is one of the five dimeric proteins belonging to the PDGF family. Other members are homodimers PDGF-BB, PDGF-CC, PDGF-DD, and the heterodimer PDGF-AB. The PDGFs are produced by platelets, stored in platelet alpha-granules and are released upon platelet activation. PDGF proteins are generally considered as potent mitogens for connective tissue cells, and can also be chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. PDGF-AA is well known as a potent mitogen for the proliferation of oligodendrocyte progenitor cells (OPCs). Two receptors have been identified for the PDGF proteins – PDGFR alpha and PDGFR beta. PDGF-AA binds to PDGFR alpha, but not PDGFR beta which appears to be specific for PDGF-BB and PDGF-AB.
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Platelet-derived growth factor-AA (PDGF-AA) is one of the five dimeric proteins belonging to the PDGF family. Other members are homodimers PDGF-BB, PDGF-CC, PDGF-DD, and the heterodimer PDGF-AB. The PDGFs are produced by platelets, stored in platelet alpha-granules and are released upon platelet activation. PDGF proteins are generally considered as potent mitogens for connective tissue cells, and can also be chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. PDGF-AA is well known as a potent mitogen for the proliferation of oligodendrocyte progenitor cells (OPCs). Two receptors have been identified for the PDGF proteins – PDGFR alpha and PDGFR beta. PDGF-AA binds to PDGFR alpha, but not PDGFR beta which appears to be specific for PDGF-BB and PDGF-AB.
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Product Details

Name Recombinant Human PDGF-AA (Carrier-free)
Cat. No. 21-7080
Alternative Names Platelet-Derived Growth Factor-AA, Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF)
Amino Acid Sequence SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity The ED50 as determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells is ≤ 1 ng/ml, corresponding to a specific activity of ≥≥≤ 1 x 106 units/mg.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant human PDGF-AA is a 28.5 kDa disulfide-linked homodimer of two A chains (250 total amino acids).
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source E. coli
Reactivity Human
Research Areas Angiogenesis/Cardiovascular; Bone, Skeletal, Cartilage; Cancer; Immune System; Neurobiology; Stem Cells & Differentiation; Wound Healing
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.