Recombinant Human Noggin (Carrier-free)
  • Recombinant Human Noggin (Carrier-free)

Recombinant Human Noggin (Carrier-free)

Cat No. Size Price Quantity
21-7075-U005 5 µg $76.00
21-7075-U020 20 µg $175.00
21-7075-U100 100 µg $725.00
21-7075-U500 500 µg $2,295.00
Lead time: 2-3 business days

Description

Noggin is a highly conserved molecule and is a secreted protein that drives neural formation and dorsalization during embryonic development. It is also a BMP antagonist, biding BMP-4 as well as BMP-2 and BMP-7, to prevent them from associating with their receptors. Noggin is expressed in defined areas of the adult central nervous system and in various peripheral tissues such as lung, skeletal muscle, and skin.

 

Recent Citations: 

Strelez C, Chilakala S, Ghaffarian K, Lau R, Spiller E, Ung N, Hixon D, Yoon AY, Sun RX, Lenz HJ, Katz JE, Mumenthaler SM. Human colorectal cancer-on-chip model to study the microenvironmental influence on early metastatic spread. iScience. 2021 May 2;24(5):102509. doi: 10.1016/j.isci.2021.102509. PMID: 34113836; PMCID: PMC8169959.

» View on PubMed.

Kim S, Choung S, Sun RX, Ung N1, Hashemi N, Fong EJ, Lau R, Spiller E, Gasho J, Foo J, Mumenthaler SM. Comparison of Cell and Organoid-Level Analysis of Patient- Derived 3D Organoids to Evaluate Tumor Cell Growth Dynamics and Drug Response. SLAS Discov. 2020 Apr 30:2472555220915827. doi: 10.1177/2472555220915827 

» View on PubMed

 

 

Name Recombinant Human Noggin (Carrier-free)
Cat. No. 21-7075
Alternative Names SYM1, SYNS, NOG
Amino Acid Sequence QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSC
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is 2.0-3.0 ng/ml of Noggin.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant human Noggin is a 46 kDa disulfide-linked homodimer (120-10C) consisting of two 206 amino acid polypeptide chains. Monomeric glycosylated Noggin migrates at an apparent molecular weight of approximately 28.0-33.0 kDa by SDS PAGE analysis under
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 95
Source HEK293 cells
Reactivity Human
Research Areas Bone, Skeletal, Cartilage; Stem Cells & Differentiation
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay

 

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Noggin is a highly conserved molecule and is a secreted protein that drives neural formation and dorsalization during embryonic development. It is also a BMP antagonist, biding BMP-4 as well as BMP-2 and BMP-7, to prevent them from associating with their receptors. Noggin is expressed in defined areas of the adult central nervous system and in various peripheral tissues such as lung, skeletal muscle, and skin.

 

Recent Citations: 

Strelez C, Chilakala S, Ghaffarian K, Lau R, Spiller E, Ung N, Hixon D, Yoon AY, Sun RX, Lenz HJ, Katz JE, Mumenthaler SM. Human colorectal cancer-on-chip model to study the microenvironmental influence on early metastatic spread. iScience. 2021 May 2;24(5):102509. doi: 10.1016/j.isci.2021.102509. PMID: 34113836; PMCID: PMC8169959.

» View on PubMed.

Kim S, Choung S, Sun RX, Ung N1, Hashemi N, Fong EJ, Lau R, Spiller E, Gasho J, Foo J, Mumenthaler SM. Comparison of Cell and Organoid-Level Analysis of Patient- Derived 3D Organoids to Evaluate Tumor Cell Growth Dynamics and Drug Response. SLAS Discov. 2020 Apr 30:2472555220915827. doi: 10.1177/2472555220915827 

» View on PubMed

 

 

Name Recombinant Human Noggin (Carrier-free)
Cat. No. 21-7075
Alternative Names SYM1, SYNS, NOG
Amino Acid Sequence QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSC
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is 2.0-3.0 ng/ml of Noggin.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant human Noggin is a 46 kDa disulfide-linked homodimer (120-10C) consisting of two 206 amino acid polypeptide chains. Monomeric glycosylated Noggin migrates at an apparent molecular weight of approximately 28.0-33.0 kDa by SDS PAGE analysis under
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 95
Source HEK293 cells
Reactivity Human
Research Areas Bone, Skeletal, Cartilage; Stem Cells & Differentiation
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay

 

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Product Details

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.