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Recombinant Human MPF (Carrier-free)
  • Recombinant Human MPF (Carrier-free)

Recombinant Human MPF (Carrier-free)

Cat No. Size Price Quantity
21-9228-U010 10 µg $70.00
21-9228-U050 50 µg $180.00
21-9228-U100 100 µg $255.00
21-9228-U250 250 µg $499.00
21-9228-U500 500 µg $825.00
21-9228-M001 1 mg $1,400.00

Description

Mesothelin is a glycosylphosphatidylinositol (GPI)-anchored, cell-surface glycoprotein predominantly secreted by cells of the mesothelium. Proteolytic cleavage of Mesothelin yields a soluble, polypeptide fragment designated megakaryocyte potentiating factor (MPF). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation in mouse bone marrow cell cultures. Mesothelin is also found overexpressed in various cancers, including malignant mesothelioma, pancreatic or ovarian carcinoma, sarcomas, and some gastrointestinal or pulmonary carcinomas. As a result of its limited expression in normal tissues, mesothelin has been reported as a useful tumor-associated marker. Recombinant Human MPF is the 250 amino acid-length fragment of the Mesothelin sequence and has a calculated molecular weight of approximately 26.7 kDa. As a result of glycosylation, Recombinant Human MPF migrates with an apparent molecular mass of approximately 32-33 kDa by SDS-PAGE gel, under reducing and non-reducing conditions.
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Mesothelin is a glycosylphosphatidylinositol (GPI)-anchored, cell-surface glycoprotein predominantly secreted by cells of the mesothelium. Proteolytic cleavage of Mesothelin yields a soluble, polypeptide fragment designated megakaryocyte potentiating factor (MPF). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation in mouse bone marrow cell cultures. Mesothelin is also found overexpressed in various cancers, including malignant mesothelioma, pancreatic or ovarian carcinoma, sarcomas, and some gastrointestinal or pulmonary carcinomas. As a result of its limited expression in normal tissues, mesothelin has been reported as a useful tumor-associated marker. Recombinant Human MPF is the 250 amino acid-length fragment of the Mesothelin sequence and has a calculated molecular weight of approximately 26.7 kDa. As a result of glycosylation, Recombinant Human MPF migrates with an apparent molecular mass of approximately 32-33 kDa by SDS-PAGE gel, under reducing and non-reducing conditions.
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Product Details

Name Recombinant Human MPF (Carrier-free)
Cat. No. 21-9228
Alternative Names Mesothelin, MSLN, SMRP, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor
Amino Acid Sequence LAGETGQEAA PLDGVLANPP NISSLSPRQL LGFPCAEVSG LSTERVRELA VALAQKNVKL STEQLRCLAH RLSEPPEDLD ALPLDLLLFL NPDAFSGPQA CTRFFSRITK ANVDLLPRGA PERQRLLPAA LACWGVRGSL LSEADVRALG GLACDLPGRF VAESAEVLLP RLVSCPGPLD QDQQEAARAA LQGGGPPYGP PSTWSVSTMD ALRGLLPVLG QPIIRSIPQG IVAAWRQRSS RDPSWRQPER
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity N/A
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass 26.7 kDa
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 95
Source CHO
Reactivity Human X
Research Areas Cancer, Stem Cells & Differentiation
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.