Recombinant Human MMP-2 (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-7072-U002 | 2 µg | $69.00 | ||
| 21-7072-U010 | 10 µg | $179.00 | ||
| 21-7072-U100 | 100 µg | $955.00 | ||
| 21-7072-U500 | 500 µg | $2,860.00 |
Technical Data Sheet
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Matrix Metalloproteinase-2 (MMP-2) belongs to the matrix metalloproteinase family of endoproteases that function in the breakdown of extracellular matrix (ECM) in various normal and disease processes. MMP-2 targets type IV collagen, as well as type V, VII and X collagens, aggrecan, link protein, elastin and fibronectin. MMP2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. MMP expression and activity are tightly regulated, both through the need to activate the inactive zymogen, and control through tissue inhibitors of matrix metalloproteinases (TIMPs).
Matrix Metalloproteinase-2 (MMP-2) belongs to the matrix metalloproteinase family of endoproteases that function in the breakdown of extracellular matrix (ECM) in various normal and disease processes. MMP-2 targets type IV collagen, as well as type V, VII and X collagens, aggrecan, link protein, elastin and fibronectin. MMP2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. MMP expression and activity are tightly regulated, both through the need to activate the inactive zymogen, and control through tissue inhibitors of matrix metalloproteinases (TIMPs).
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Product Details
| Name | Recombinant Human MMP-2 (Carrier-free) |
|---|---|
| Cat. No. | 21-7072 |
| Alternative Names | Matrix metalloproteinase-2; Gelatinase A, TBE-1 |
| Amino Acid Sequence | MYNFFPRKPK WDKNQITYRI IGYTPDLDPE TVDDAFARAF QVWSDVTPLR FSRIHDGEAD IMINFGRWEH GDGYPFDGKD GLLAHAFAPGT GVGGDSHFDDD ELWTLGEGQV VRVKYGNADGE YCKFPFLFNGK EYNSCTDTGRS DGFLWCSTTYNF EKDGKYGFCPHE ALFTMGGNAEG QPCKFPFRFQ GTSYDSCTTE GRTDGYRWCG TTEDYDRDKKY GFCPETAMST VG |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | MMP-2 activity was measured by its ability to cleave a chromogenic peptide MMP-2 substrate at room temperature. At an MMP-2 concentration of ≥ 2.5 µg/ml, 50% cleavage was achieved at an incubation time of approximately 25 minutes. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant human MMP-2 is a 62.0 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain (552 amino acids). |
| Protein Content | Content Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 90 |
| Source | E. coli |
| Reactivity | Human |
| Research Areas | Angiogenesis/Cardiovascular; Bone, Skeletal, Cartilage; Cancer; Wound Healing |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.