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Recombinant Human MIF (Carrier-free)
  • Recombinant Human MIF (Carrier-free)

Recombinant Human MIF (Carrier-free)

Cat No. Size Price Quantity
21-8500-U005 5 µg $69.00
21-8500-U025 25 µg $175.00
21-8500-U100 100 µg $555.00
21-8500-U500 500 µg $1,610.00

Description

MIF (or macrophage migration inhibitory factor) is a small secreted protein that has both cytokine (as an inflammatory mediator) and enzyme (tautomerase activity) properties. MIF induces inflammatory cytokines, nitric oxide and superoxide anions, and regulates macrophage and lymphocyte proliferation, apparently signaling through CD74 and CD44. MIF is widely expressed in a variety of tissues and many cell lines.
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MIF (or macrophage migration inhibitory factor) is a small secreted protein that has both cytokine (as an inflammatory mediator) and enzyme (tautomerase activity) properties. MIF induces inflammatory cytokines, nitric oxide and superoxide anions, and regulates macrophage and lymphocyte proliferation, apparently signaling through CD74 and CD44. MIF is widely expressed in a variety of tissues and many cell lines.
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Product Details

Name Recombinant Human MIF (Carrier-free)
Cat. No. 21-8500
Alternative Names Macrophage Migration, Inhibitory Factor, GLIF, MMIF, GIF, Glycosylation-inhibiting factor
Amino Acid Sequence HHHHHHHHAM PMFIVNTNVP RASVPDGFLS ELTQQLAQAT GKPPQYIAVH VVPDQLMAFG GSSEPCALCS LHSIGKIGGA QNRSYSKLLC GLLAERLRIS PDRVYINYYD MNAANVGWNN STFA
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Determined by its ability to inhibit monocyte migration.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Insect cell derived recombinant MIF is a 15 kDa protein containing 124 amino acid residues, including an N-terminal His-tag.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source Hi-5 Insect cells
Reactivity Human
Research Areas Chemotaxis; Inflammation
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.