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Recombinant Human MANF (Carrier-free)
  • Recombinant Human MANF (Carrier-free)

Recombinant Human MANF (Carrier-free)

Cat No. Size Price Quantity
21-7111-U005 5 µg $76.00
21-7111-U025 25 µg $175.00
21-7111-U100 100 µg $585.00
21-7111-U500 500 µg $1,795.00

Description

Mesencephalic astrocyte-derived neurotrophic factor (MANF), also known as ARMET or ARP, is a secreted protein belonging to the ARMET family. It is localized to the Golgi apparatus and the endoplasmic reticulum (ER), and has been reported to play a role in modulating myocardial damage from ER stress and has been called a cardiomyokine. MANF, like its structural homolog CDNF, has been described as a survival factor for midbrain dopaminergic neurons in vitro and in vivo.
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Mesencephalic astrocyte-derived neurotrophic factor (MANF), also known as ARMET or ARP, is a secreted protein belonging to the ARMET family. It is localized to the Golgi apparatus and the endoplasmic reticulum (ER), and has been reported to play a role in modulating myocardial damage from ER stress and has been called a cardiomyokine. MANF, like its structural homolog CDNF, has been described as a survival factor for midbrain dopaminergic neurons in vitro and in vivo.
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Product Details

Name Recombinant Human MANF (Carrier-free)
Cat. No. 21-7111
Alternative Names Mesencephalic Astrocyte-derived Neurotrophic Factor, ARMET, Arginine-rich protein (ARP), mutated in early stage tumors
Amino Acid Sequence LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity The expected ED50 for this effect is 15-25 μg/g, based on its ability to stimulate the proliferation of rat C6 cells.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human MANF comprises 8 cysteine residues and consists of 158 amino acids. It has a molecular weight of approximately 18.1 kDa.
Protein Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source E. coli
Reactivity Human
Research Areas Neurobiology
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.