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Recombinant Human LIF (Carrier-free)
  • Recombinant Human LIF (Carrier-free)

Recombinant Human LIF (Carrier-free)

Cat No. Size Price Quantity
21-8460-U005 5 µg $69.00
21-8460-U025 25 µg $179.00
21-8460-U100 100 µg $625.00
21-8460-U500 500 µg $1,525.00

Description

Leukemia Inhibitory Factor (LIF) is a member of the IL-6 family of cytokines, based on its helical structure. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. Expression has also been reported in T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation, however inhibition appears to be mouse specific. LIF can be upregulated by proinflammatory cytokines such as IL-17 and TNF-alpha. It has also been reported to promote regulatory T cell and inhibit Th17 cell differentiation.
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Leukemia Inhibitory Factor (LIF) is a member of the IL-6 family of cytokines, based on its helical structure. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. Expression has also been reported in T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation, however inhibition appears to be mouse specific. LIF can be upregulated by proinflammatory cytokines such as IL-17 and TNF-alpha. It has also been reported to promote regulatory T cell and inhibit Th17 cell differentiation.
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Product Details

Name Recombinant Human LIF (Carrier-free)
Cat. No. 21-8460
Alternative Names Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor (MLPLI), Leukemia Inhibitory Factor
Amino Acid Sequence SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant human LIF is a 19.6 kDa protein containing 180 amino acids residues including three disulfide bonds.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source E. coli
Reactivity Human
Research Areas Diabetes / Weight Regulation; Immune System; Lipid Metabolism; Proliferation; Stem Cells & Differentiation; Neurobiology
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.