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Recombinant Human Klotho (Carrier-Free)
  • Recombinant Human Klotho (Carrier-Free)

Recombinant Human Klotho (Carrier-Free)

Cat No. Size Price Quantity
21-9048-U005 5 µg $80.00
21-9048-U020 20 µg $195.00
21-9048-U100 100 µg $600.00
21-9048-U500 500 µg $1,800.00

Description

Klotho is a glycosylated protein that plays an important role in the regulation of phosphate and calcium homeostasis. Human Klotho exists in both membrane bound and secreted forms, and is predominantly expressed in the kidney convoluted tubules, and, to a lesser extent, in the brain, reproductive organs, endocrine glands, urinary bladder, skeletal muscle, placenta, and colon. Recombinant Human Klotho is a glycoprotein of ≥ 516 amino acid residues that migrates at an apparent molecular weight of 65-70 kDa by SDS-PAGE analysis under reducing conditions.
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Klotho is a glycosylated protein that plays an important role in the regulation of phosphate and calcium homeostasis. Human Klotho exists in both membrane bound and secreted forms, and is predominantly expressed in the kidney convoluted tubules, and, to a lesser extent, in the brain, reproductive organs, endocrine glands, urinary bladder, skeletal muscle, placenta, and colon. Recombinant Human Klotho is a glycoprotein of ≥ 516 amino acid residues that migrates at an apparent molecular weight of 65-70 kDa by SDS-PAGE analysis under reducing conditions.
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Product Details

Name Recombinant Human Klotho (Carrier-Free)
Cat. No. 21-9048
Alternative Names Alpha-klotho, KLA
Amino Acid Sequence EPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGGWQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD VASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGLRYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DHFRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSPRL GY
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Determined by the dose-dependent stimulation of the proliferation of Mouse NIH-3T3 cells. Recombinant Human Klotho is effective in a concentration range of 0.5-2.0 ug/ml.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human Klotho has a calculated molecular weight of ≥ 58.6 kDa.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source CHO cells
Reactivity Human
Research Areas Proliferation, Stem Cells & Differentiation, Angiogenesis/Cardiovascular, Bones, Skeletal, Cartilage
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.