Recombinant Human Klotho (Carrier-Free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-9048-U005 | 5 µg | $80.00 | ||
| 21-9048-U020 | 20 µg | $195.00 | ||
| 21-9048-U100 | 100 µg | $600.00 | ||
| 21-9048-U500 | 500 µg | $1,800.00 |
Technical Data Sheet
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Klotho is a glycosylated protein that plays an important role in the regulation of phosphate and calcium homeostasis. Human Klotho exists in both membrane bound and secreted forms, and is predominantly expressed in the kidney convoluted tubules, and, to a lesser extent, in the brain, reproductive organs, endocrine glands, urinary bladder, skeletal muscle, placenta, and colon. Recombinant Human Klotho is a glycoprotein of ≥ 516 amino acid residues that migrates at an apparent molecular weight of 65-70 kDa by SDS-PAGE analysis under reducing conditions.
Klotho is a glycosylated protein that plays an important role in the regulation of phosphate and calcium homeostasis. Human Klotho exists in both membrane bound and secreted forms, and is predominantly expressed in the kidney convoluted tubules, and, to a lesser extent, in the brain, reproductive organs, endocrine glands, urinary bladder, skeletal muscle, placenta, and colon. Recombinant Human Klotho is a glycoprotein of ≥ 516 amino acid residues that migrates at an apparent molecular weight of 65-70 kDa by SDS-PAGE analysis under reducing conditions.
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Product Details
| Name | Recombinant Human Klotho (Carrier-Free) |
|---|---|
| Cat. No. | 21-9048 |
| Alternative Names | Alpha-klotho, KLA |
| Amino Acid Sequence | EPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGGWQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD VASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGLRYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DHFRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSPRL GY |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | Determined by the dose-dependent stimulation of the proliferation of Mouse NIH-3T3 cells. Recombinant Human Klotho is effective in a concentration range of 0.5-2.0 ug/ml. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant Human Klotho has a calculated molecular weight of ≥ 58.6 kDa. |
| Protein Content | Content Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 98 |
| Source | CHO cells |
| Reactivity | Human |
| Research Areas | Proliferation, Stem Cells & Differentiation, Angiogenesis/Cardiovascular, Bones, Skeletal, Cartilage |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.