Recombinant Human INSL5/INSL7 Hybrid (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-7110-U005 | 5 µg | $76.00 | ||
| 21-7110-U025 | 25 µg | $175.00 | ||
| 21-7110-U100 | 100 µg | $585.00 | ||
| 21-7110-U500 | 500 µg | $1,795.00 |
Technical Data Sheet
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At least seven relaxin-like proteins make up the Insulin/Relaxin superfamily. Most consist of two peptide subunits, an alpha-chain and a beta-chain, linked by disulfide bonds. INSL5 has been identified as the cognate ligand for the G-protein-coupled receptor GPCR142 (RXFP4). INSL7 (Relaxin-3) has been reported as a ligand for LGR7, as well as for GPCR135 and GPCR142. Sequence analysis indicates that INSL5 and INSL7 are highly homologous, but INSL5 seems to be specific only to GPCR142. The recombinant INSL5/INSL7 hybrid protein can activate the G protein coupled receptors GPCR135 and GPCR142, but unlike INSL7, does not induce significant activation of the LGR7 receptor.
At least seven relaxin-like proteins make up the Insulin/Relaxin superfamily. Most consist of two peptide subunits, an alpha-chain and a beta-chain, linked by disulfide bonds. INSL5 has been identified as the cognate ligand for the G-protein-coupled receptor GPCR142 (RXFP4). INSL7 (Relaxin-3) has been reported as a ligand for LGR7, as well as for GPCR135 and GPCR142. Sequence analysis indicates that INSL5 and INSL7 are highly homologous, but INSL5 seems to be specific only to GPCR142. The recombinant INSL5/INSL7 hybrid protein can activate the G protein coupled receptors GPCR135 and GPCR142, but unlike INSL7, does not induce significant activation of the LGR7 receptor.
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Product Details
| Name | Recombinant Human INSL5/INSL7 Hybrid (Carrier-free) |
|---|---|
| Cat. No. | 21-7110 |
| Alternative Names | H3, Relaxin-3, RXN3, ZINS4, insulin-like peptide INSL5, prepro-INSL5 |
| Amino Acid Sequence | A-chain: QDLQTLCCTD GCSMTDLSAL C B-chain: RAAPYGVRLC GREFIRAVIF TCGGSRW |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | Data not available. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | The Recombinant Human INSL5/INSL7 hybrid consists of a 21 amino acid alpha-chain (INSL5) linked to a 27 amino acid beta-chain (INSL7) by two disulfide bonds and has a molecular weight of ≥ 5.2 kDa. |
| Protein Content | Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 98 |
| Source | E. coli |
| Reactivity | Human |
| Research Areas | Diabetes, Lipid Metabolism, Neurobiology |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.